AGBT 2012 Tweets Day 3

17 Feb 2012

(Day 1, Day 2, Day 3, Day 4)

AGBT has been low on announcements so far. As Dan Koboldt says people are now a bit more focused on how to interpret all that lovely data they've been generating. Probably no bad thing, but that's not what we want from AGBT. We want buzz, hype, outrageous marketing statements and speculation on the future of sequencing.

Today my prayers may be answered ... all eyes will be on Clive Brown and Oxford Nanopore at 11:40am local (4:40pm GMT).

Scientific agenda

9:00-9:35 am, Keynote Speaker: Rick Myers, HudsonAlpha Institute for Biotechnology, “Genetics and Epigenetics of Human Gene Regulation”
9:35-10:05 am, Tom Gingeras, Cold Spring Harbor Laboratory, “Important Lessons from Complex Genomes”
10:05-10:25 am, Geoffrey Smith, Illumina Cambridge Ltd., “The Miseq DNA Sequencing Platform and Application in Clinical Microbiology”
11:00-11:20 am, Joseph Boland, National Cancer Institute, “Exome Sequencing on the PGM: Exome in a Day”
11:20-11:40 am, Richard Roberts, New England Biolabs, “Characterization of DNA Methyltransferase Specificities Using Single-Molecule, Real-Time DNA Sequencing”
11:40 am-12:00 pm, Clive Brown, Oxford Nanopore Technologies, “Single Molecule ‘Strand’ Sequencing Using Protein Nanopores and Scalable Electronic Devices”
12:15-2:15 pm, Pacific Biosciences Workshop
2:15-2:35 pm, Illumina, Inc., Elliott Margulies, “From Sample to Answer: Fast and Accurate Genome Sequencing for the Clinic”
2:35-2:55 pm, Caliper a Perkin Elmer Company, Kevin Hrusovsky, “Next Generation Technologies Enabling a Revolution in Medicine”
2:55-3:15 pm, Complete Genomics, Carlos Bustamante, Stanford University, “Population Genetic Inference in the Personal Era”
3:15-3:35 pm, RainDance Technologies, Andy Watson, “Recent Publication Successes: The Power of RainDance Targeted Sequencing”
3:35-3:55 pm, Advanced Analytical, Steve Siembieda, “Fragment Analyzer™- Streamlining Quality and Quantity Analysis of Nucleic Acids”
3:55-4:15 pm, NuGen Technologies, Inc., Elaine Mardis, Washington University School of Medicine, “Simplifying Genomic Sample Preparation with Digital Microfluidics”

Evening session topics

Cancer and Transcriptomes (Michael Metzker, Baylor College of Medicine, Chair)
Genome Biology (Steven Jones, British Columbia Cancer Agency, Chair)
Computational Biology (Mike Zody, Broad Institute of MIT and Harvard, Chair)

Most popular tweets
(34) nanopore: Introducing the MinION, a nanopore sequencer the size of a USB stick. Works just like GridION, but smaller #AGBT
(22) pathogenomenick: Oxford Nanopore megaton announcement: Why do you need a machine? exclusive interview on my blog! http://t.co/tJxQit9T #AGBT
(22) kbradnam: Reactions to @nanopore news. Biologists: cheer. Bioinformaticians: groan. System admins: weep. #AGBT
(20) bioitworld: Full story on Oxford @nanopore stunning presentation from CTO Clive Brown at #AGBT http://t.co/VNvCxLQN
(17) Copenhagenomics: Here's a spy photo from a projected sequencing center, planned to open in early 2013: http://t.co/BjaSNe1P #AGBT
(17) Matt_Francis: #AGBT obi-wan:I felt a great disturbance in the force, as if a million #illumina investors cried out in pain #oxford #Nanopore
(13) nanopore: of course! RT @kbradnam: @nanopore release also reveals that MinION syncs with iTunes, acts as a 3G modem, and has a fresh lemon scent #AGBT
(13) genomesunzipped: Making sequencing simpler with nanopores: Our take on the new Oxford @Nanopore machines #AGBT http://t.co/cjrQX1db
(12) kbradnam: Things to tell your grandchildren: "and we used to work in large buildings called sequencing centers" #AGBT
(10) pathogenomenick: The full Oxford Nanopore press release up here http://t.co/JnJHjsD4 #AGBT
(9) genetics_blog: If looking through the #AGBT tag is too daunting, @pathogenomenick is doing a fine job summarizing at http://t.co/CWWf79Yi. Thank you sir
(9) pathogenomenick: I've embedded all the nanopore videos on the blog here http://t.co/rhBpP9XS #AGBT
(9) acdarby: I am a bit worried that my computer does not have enough USB ports to turn it into a sequencing lab #AGBT @nanopore!
(7) girlscientist: Rich Roberts plays to crowd by asking if some of money being spent on DNA sequencing could instead be used figuring out what genes do. #AGBT
(6) girlscientist: Myers: analysis of DNA methylation in family shows genetics have strong effect, http://t.co/TxLlZIle #AGBT
(6) ElementoLab: #AGBT Tom Gingeras: extremely low copy number transcripts in 1 cell type can be highly expressed in another cell type
(6) timjph: New sequencers announced at #AGBT by Oxford Nanopore; one USB sized @pathogenomenick: full press release here http://t.co/fYNWJYEf #AGBT
(5) ElementoLab: #AGBT Tom Gingeras: intergenic regions migth be much smaller than currently thought, genes might massively overlap
(5) girlscientist: Eric Green asks Geoffrey Smith of Illumina the $1M question: where do you actually see MiSeq's being deployed? A: in clinics, we hope #AGBT
(5) bmahersciwriter: RT @girlscientist: Eric Green asks Illumina the $1M question: where do you actually see MiSeq's being deployed? A: in clinics, we hope #AGBT
(5) Massgenomics: Joseph Boland on IonTorrent PGM "Exome in a Day". Sure, if you ignore the fact that hybridization takes 3 days. #AGBT.
(5) deannachurch: RR: There is a lot of money being spent on sequencing- it would be nice to see some of that spent on understanding what genes do. #AGBT
(5) Copenhagenomics: Richard Roberts: "Can we use a fraction of the $$$ we spend on sequencing, to actually find out what the genes are doing?" #applause #AGBT
(5) ElementoLab: #AGBT Clive Brown: RNA can be DIRECTLY read using nanopores - no cDNA
(5) notSoJunkDNA: So, it's for real? DNA sequencer the size of a USB key: http://t.co/VSOyO4WD #AGBT (via @nanopore )
(5) illumina: Loman and Robison on #AGBT big news, the MiniIon http://t.co/EUYEpp1d and http://t.co/iGRNR6gq
(5) leonidkruglyak: Great problem to have: "how will 100kb+ reads change your research?" http://t.co/5A6wlkfc #AGBT
(5) SEQanswers: Realists: spit take. @kbradnam: Reactions to @nanopore news. Biologists: cheer. Bioinformaticians: groan. System admins: weep. #AGBT
(5) kbradnam: Warning to all employees of @Nanopore. If you carry any 'regular' USB flash drives on you, prepare to be mugged by rabid genomicists #AGBT
(4) girlscientist: Come tweet my talk at 730 tonight #AGBT, Salon E/F: you will learn about the awesome power of immunogenomics (*hype included w/no charge)!

5 - 6am EST
+5m archivist84: Question to those at #AGBT - After we sort the #datadeluge will we have to tackle an #informationinundation ???
+9m t_basi: Nice blog that even I can understand: #AGBT Marco Island Never Gets Old http://t.co/QrvhInBo
+25m lucamartins12: @begandara p mlk agbt vai aloprar
+34m ewanbirney: I went past 1000 followers yesterday - no doubt to AGBT - am and now at the round number of 1011. Welcome!
+47m pathogenomenick: #AGBT day 3 tweets kick off here http://t.co/RfDEXv7H - today's the big one!

6 - 7am EST
+4m omespeak: RT @pathogenomenick All of the #AGBT day 2 tweets are available at http://t.co/1XkWv6XH
+5m pathogenomenick: A bit of info on GnuBIO and Lasergen announcements http://t.co/wmy0taIZ #AGBT - both sound pretty interesting!
+19m mcaccamo: RT @GenomeAnalysis: if you are at #AGBT remember at 16:30 - 18:00 today Rob Davey will be presenting MISO - An Open Source LIMS - Poster 71
+29m hiro_h: AGBT tweets 2012 (1) http://t.co/Gn5LFZMN

7 - 8am EST
+7m pascal_biotech: Good report on AGBT 2012 Day 1: Clinical Annotation and Fierce Competition http://t.co/shuHIlKH #agbt
+11m engawa_02: AGBT tweets 2012 (1) http://t.co/AaFMlZYq
+21m girlscientist: Come tweet my talk at 730 tonight #AGBT, Salon E/F: you will learn about the awesome power of immunogenomics (*hype included w/no charge)!

8 - 9am EST
+24m Copenhagenomics: RT @rforsberg: Europe also has a genomics conference inspired by #AGBT - it's called @Copenhagenomics - http://t.co/FJrXPacO #CPHx
+24m illumina: New blog post from Live at #AGBT http://t.co/isP5PzDn
+39m GenomeScience: via @girlscientist at #AGBT Good q at @PacBio event: who's going to pay for the storage of data we all want to generate?
+45m illumina: On Facebook? Check out pictures from #AGBT on our page. http://t.co/dQC1dUKE
+59m omespeak: RT @illumina: New blog post from Live at #AGBT http://t.co/f8Cn91ky

9 - 10am EST
+0m fluidigm: Suite 285 at #AGBT, last day for drop in discussions, 9am -5pm, Single-Cell Gene Expression, Targeted Resequencing and more>o
+0m omespeak: We had GnuBio and LaserGen announcing new sequencing machines last night. Today, Oxford Nanopore makes big announcement. #AGBT preview
+1m EdgeBio: Good Morning! Here are some more live updates via @dgaalaas on some of the talks from Life Tech and GNUbio #AGBT http://t.co/yQV86960
+3m djschlesinger: First talk of morning session: Rick Myers from HudsonAlpha talking about genetics and epigenetics of gene regulation #AGBT
+3m deannachurch: Good morning! Up first Rick Myers on genetics and epigenetics of human gene regulation. #AGBT
+3m CompleteGenomic: Come see Steve Lincoln, our VP of scientific apps, present on our assembly process in the #AGBT Complete Suite today at 10:30 am
+5m bffo: RM has been working on transcription gene regulation for 30 years ... Should be a great talk! #agbt
+5m girlscientist: Day 2 of #AGBT starts with Rick Myers of @HudsonAlpha. That's my boss up there, so I expect only good tweets from y'all.
+5m bioinfosm: #agbt tweeps, wassup with the tweetup; when and where? #anotherParty
+6m girlscientist: .@bioinfosm #AGBT tweetup is in hotel lobby bar at 9:30ish after sessions end tonight.
+7m bffo: C U all there! RT @girlscientist: .@bioinfosm #AGBT tweetup is in hotel lobby bar at 9:30ish after sessions end tonight.
+9m girlscientist: R Myers echoes theme of meeting: you learn a lot more when you integrate a number of different data types, as in ENCODE. #AGBT
+9m ElementoLab: #AGBT Rick Myers: we will learn a lot by integrating interactome, transcriptome, methylome and allelic variation
+12m bffo: RM:Encode neophytes should look at the guide paper, from PLoS Biology paper http://t.co/gvTjRKCf #AGBT
+14m illumina: Rick Myers, HudsonAlpha Inst, Encode: Integrate data from interactome, transcriptome, methylome with allelic variation #AGBT
+14m ElementoLab: #AGBT Rick Myers: has performed ChIP-seq for ~80 transcription factors in many cell lines for ENCODE
+14m deannachurch: RM: analyzed over 80 transcription factors as part of ENCODE #AGBT
+15m dgaalaas: Good morning. Rick Myers from Hudson Alpha talking ENCODE and ChIP-Seq at #AGBT
+16m illumina: Myers: NSRF is almost exclusively a repressor, binds ~2000 sites. Could not find NRSF binding site upstr of neuro D, until ChIP-Seq #AGBT
+16m deannachurch: RM: by integrating binding data with variation data they can find both allele biased ans allele specific binding. #AGBT
+17m ElementoLab: #AGBT Rick Myers: found a lot of allele-specific binding
+17m finchtalk: #AGBT Rick Meyers on the gene regulation and the ENCODE project. Allele specific expression is wide spread.
+18m illumina: Myers: ChIP-seq on over 80 TFs many cell lines. Allele specific occupancy of protein on DNA & only G allele bound by GABP #AGBT
+19m ElementoLab: #AGBT Rick Myers: have developed transposon mediated method to make RNA-seq libraries, Gertz et al, 2012, Genome Research
+19m ElementoLab: #AGBT Rick Myers: can work with very small quantities of RNA, down to 1ng
+20m girlscientist: Myers: recent paper on transposase-mediated construction of RNA-seq libraries, in @genomeresearch: http://t.co/1lpB7JY0 #AGBT
+20m finchtalk: #AGBT RM on RNA-seq, alignment and gene models are an unsolved problem. Transposon based "shearing" improves lib prep.
+20m illumina: Myers: w/RNA-seq 40M reads gives 40 reads for 1 kb. really really sensitive #AGBT
+21m omespeak: Rick Myers mentions difficulty of sequencing methylation using bisulfite (too expensive now). Perhaps nanopores will change that? #AGBT
+21m illumina: Myers RNA seq library contruction by transposon hopping, much more easier and quite sensitive only 10 pg mRNA #AGBT
+21m rnomics: RT @finchtalk: #AGBT RM on #RNA-seq, alignment and gene models are an unsolved problem.... http://t.co/ZJtnjjfa #rnomics-bioscience @MyEN
+22m ElementoLab: #AGBT Rick Myers: did RRBS on 82 cell lines and tissues for ENCODE, clearly cell lines massively hypermethylated compared to tissues
+22m GenomeBiology: DNA methylation profiling of 82 cell lines and tissues on encode project. RM #agbt
+23m illumina: Myers: DNA methylation, whole genome bisulfite expensive. Compromise = RRBS, w/ Illumina 450k array. Very sensitive, quantitative #AGBT
+23m EpicentreBio: RT @pathogenomenick: #AGBT blog-post round-up http://t.co/p3yUQSPV
+23m girlscientist: Myers: analysis of DNA methylation in family shows genetics have strong effect, http://t.co/TxLlZIle #AGBT
+23m NCBI: This afternoon at Poster 59, @deannachurch will discuss the impact of structural variation on genome assembly. #AGBT http://t.co/1Kj0Xmpy
+24m deannachurch: RM: genetics has a strong affect on methylation patterns. #AGBT
+24m EpicentreBio: RT @illumina: On Facebook? Check out pictures from #AGBT on our page. http://t.co/nnwjLErU
+24m ElementoLab: #AGBT Rick Myers: 92% allele specific methylation is genetic, 8% is imprinting
+26m illumina: Myers: Genetics has a strong effect on methylation patterns, 8% allele-specific imprinting, 92% was genetic #AGBT
+26m ElementoLab: #AGBT Rick Myers: in clinical trial of anti-DR5 antibody in breast cancer, methylation at 114 CpGs is associated w response to therapy
+26m girlscientist: RT @NCBI: This afternoon at Poster 59, @deannachurch will discuss the impact of structural variation on genome assembly. #AGBT
+27m Massgenomics: Good lineup at #AGBT this morning: Rick Myers (HudsonAlpha), Tom Gingeras (CSHL), Geoffrey Smith (Illumina), then much-needed coffee.
+27m GenomeRef: Com to poster 59 this afternoon at #AGBT to see what we are up to with the reference assembly. #AGBT
+27m omespeak: "correlation between methylation and gene expression depends on location of CpG" Rick Myers #AGBT
+27m iontorrent: Rick Myers, Hudson Alpha, shows 114 CpGs associated with response to antibody therapy. #AGBT
+27m illumina: Myers: Correlation of methylation and gene expression depends on location of CpG #AGBT
+28m westr: +1 RT @girlscientist: Myers: analysis of DNA methylation in family shows genetics have strong effect, http://t.co/hgCK8IX7 #AGBT #pm101
+28m ElementoLab: #AGBT Rick Myers: methylation near TSSs negatively correlates w gene expression
+29m ElementoLab: #AGBT Rick Myers: gene body methylation positively correlates w gene expression EXCEPT when CpG islands in gene bodies are methylated
+30m NCBI: Tonight at 9:30 in Salon G, NCBI's Eugene Yaschenko will talk about new NGS data compression strategies. #AGBT http:http://t.co/1Kj0Xmpy
+30m ElementoLab: #AGBT Rick Myers: these gene body CGI are typically enhancers, and need to be unmethylated for gene to be transcribed
+31m NCBI: Tonight at 9:30 in Salon G, NCBI's Eugene Yaschenko will talk about new NGS data compression strategies. #AGBT http://t.co/1Kj0Xmpy
+32m GenomeBiology: Genetics meets genomics when variants correlate with phenotype RM #agbt
+32m CompleteGenomic: Great roundtable discussion this morning with Carlos Bustamante of Stanford U at #AGBT http://t.co/7fcXmUAy
+33m Gavin_Oliver: # AGBT Will keep you up to date on Oxford Nanopore announcements here: http://t.co/Rk184B0R
+33m maureen_mack: Good morning @Personal_RX_WI friends at #agbt. What's on the agenda for today?
+36m deannachurch: Now up Tom Ginger as on important lessons from complex genomes. #AGBT
+36m CRIgenomics: #ONT are talkng today at #AGBT, anyone want to place a bet on the type and number of genomes they have sequenced?
+36m bioinfosm: a real fun video (not sure of the facts yet) from #ionTorrent on homopolymer comparisons with #illumina = http://t.co/TcAfFaYQ #agbt #ngs
+36m ElementoLab: #AGBT Tom Gingeras: lessons learned from complex genomes
+37m girlscientist: Like that Tom Gingeras's computer had a calendar reminder pop up: "Your Talk = Now." #AGBT
+38m Personal_RX_WI: Day 3 #AGBT Genomic Studies and New Tech. Looking forward to Oxford Nanopore single molecule seq talk late morning. Stay tuned. . .
+38m omespeak: RT @CRIgenomics: #ONT are talkng today at #AGBT, anyone want to place a bet on the type and number of genomes they have sequenced?
+38m obahcall: Tom Hudson on lessons learned from ENCODE and modENCODE #AGBT
+40m obahcall: Tom Gingeras on lessons learned from ENCODE and modENCODE #AGBT
+40m deannachurch: TG: over 51,000 genic regions 40.5% protein coding, over 161,000 transcripts. #AGBT
+40m KamounLab: Nanopores or NanoBores? RT @Gavin_Oliver # AGBT Will keep you up to date on Oxford Nanopore announcements here: http://t.co/vbSoAWYJ
+41m deannachurch: TG: genes don't have a single control region- they have multiple. #AGBT
+42m bioinfosm: TG: 51k genes, 8 transcripts/gene on avg, 40% are protein coding; alt start could be anywhere; promoters embedded in introns as well! #agbt
+42m illumina: Gingeras, Lessons learned from complex genomes. 8 transcripts per gene locus more than half are non-coding. #AGBT
+42m bffo: RM: fEffects of sequence variation on differential allelic transcription factor occupancy & gene expression. http://t.co/qdAf8OxO #AGBT
+42m finchtalk: #AGBT Tom Gingeras on the transcriptomes: the rule, genes have multiple control points. The exception is 1 gene 1 transcript
+43m ElementoLab: #AGBT Tom Gingeras: it is critical to think that most genes have multiple transcription initiation sites
+43m michaelgrr: presentation from Oxford @nanopore at the Advances in Genome Biology and Technology (#AGBT) conf coming up at 11.40am (EST) / 4.40pm (GMT).
+43m illumina: Gingeras: Number of genes increased 41% since 2004, number of transcripts increased 52% since 2008 #AGBT
+43m timjph: Lots of GRCh37 patches for one! RT @GenomeRef: poster 59 if you're at #AGBT to see what GRC is up to with the reference assembly
+46m bioinfosm: #agbt TG: thats a fun term i learnt 'irreproducibility detection rate IDR'
+46m bioinfosm: #agbt, sorry the term is from TG, but comment is mine :)
+47m genome_gov: Signing on from the Advances in Genome Biology and Technology Conference in Marco Island, FL. Day 2 of talks. #AGBT
+47m illumina: Gingeras: cellular addresses RNA for localization: cytoplasmic, nuclear (nucleoli, nucleoplasm, chromatin) #AGBT
+47m westr: Would like to hear more about it: RT @iontorrent Rick Myers, Hudson Alpha, shows 114 CpGs associated with response to antibody therapy #AGBT
+48m ElementoLab: #AGBT Tom Gingeras: how do you know low read cnt is background or real transcription? irreproducibility detection rate (IDR) (Bickel et al)
+48m westr: RT @deannachurch: TG: over 51,000 genic regions 40.5% protein coding, over 161,000 transcripts. #AGBT // Includes ncRNA genes #pm101
+49m Massgenomics: Tom Gingeras: 80% of the genome covered by primary transcripts (90.7% of exons, 79.3% of introns, 35.5% of intergenic seqs) #AGBT.
+49m ElementoLab: #AGBT Tom Gingeras: found up to 15K NOVEL multi exon transcripts in intergenic regions, 8K antisense
+50m westr: NB! RT @ElementoLab: #AGBT Tom Gingeras: it is critical to think that most genes have multiple transcription initiation sites #pm101
+50m girlscientist: Gingeras: A very large portion of the genome is appearing in the RNA space as unspliced transcript, very often polyA- and antisense. #AGBT
+51m ElementoLab: #AGBT Tom Gingeras: (that was based on RNA-seq in 15 cell lines)
+51m NEBiolabs: Hear Rich Roberts, NEB CSO, speak at 11:20 in the Plenary Session at #AGBT. http://t.co/4LcUZpwW
+51m CRIgenomics: #AGBT Tom Gingeras IDR analysis (Li et al 2011). Statistics for two replicates, I know a lot of biologists who will say two is enough!
+52m ElementoLab: #AGBT Tom Gingeras: intergenic regions migth be much smaller than currently thought, genes might massively overlap
+52m iontorrent: @westr #AGBT cool stuff! He is going into publication now. Stay tuned.
+53m girlscientist: Gingeras elaborating on previous intriguing suggestions that the amount of transcription seen means we need to redefine "gene." #AGBT
+53m bongopondit: Actually finding it easier to follow these genomics-heavy talks through the summaries on Twitter (my background is not in genomics). #AGBT
+53m bioinfosm: #agbt have not heard of STAR; TG: rna-seq reads mapped using STAR
+53m CRIgenomics: Illumina previewed 687bp reads at #AGBT, no wonder Roche are circling. 25M on MiSeq coming soon...
+55m illumina: Illumina Talk: MiSeq DNA Seq Platform and Application in Clinical Microbiology -- starts in 10 min, Island Ballroom #AGBT
+55m ClaireAinsworth: This v cool. MT @girlscientist: Gingeras elaborating on suggestions that amount of transcription seen = we need to redefine "gene." #AGBT
+55m bongopondit: Missed this news earlier. 687bps! Holy moly! MT @CRIgenomics: Illumina previewed 687bp reads at #AGBT,
+56m iontorrent: For ea category of genes w/ 2 isoforms, both will be expressed in cells. Minor/alt isoforms seen in specific compartments -T Gingeras #AGBT
+56m omespeak: Missed this news earlier. 687bps! Holy moly! MT @CRIgenomics: Illumina previewed 687bp reads at #AGBT,
+56m girlscientist: Gingeras: most genes have multiple isoforms expressed - and these isoforms are each located in specific compartments of the cell. #AGBT
+56m illumina: Gingeras: More than half of 247,685 unannotated single exome transcripts are intergenic and antisense #AGBT
+57m ElementoLab: #AGBT Tom Gingeras: in a given cell, most isoforms of a given gene are expressed, whether the gene has 2 isoforms or 10+
+57m illumina: Gingeras: Reduction of intergenic region sizes, <10,000 bp due to the discovery of more transcripts #AGBT
+57m Massgenomics: Tom Gingeras did a 454 run looking for novel unannotated GTAG junction seqs. What was that "50% false neg rate for RNA-seq?" about? #AGBT
+57m PacBio: #AGBT PacBio Workshop today at 12:15 in Palms Ballroom. Speakers incl. Erich Jarvis (Duke), Paul Hagerman (UC Davis), Matt Waldor (Harvard)
+58m bioinfosm: #agbt Gingeras: fate of most long ncRNA is small RNA - confirmed by conservation analysis phastCons
+58m illumina: Gingreas: an indivl cell w/ multiple isoforms, some dominant & the minor ones only in specific cell compartments. Incredible! #AGBT

10 - 11am EST
+2m illumina: Gingeras: Drospolia snoRNA, only 25-30% are capped full length, and 20-40% uncapped,= a specific processing site w/ends conserved! #AGBT
+5m omespeak: Genomics papers are the new high-energy physics papers (50+ authors). #AGBT
+6m ElementoLab: #AGBT Tom Gingeras: extremely low copy number transcripts in 1 cell type can be highly expressed in another cell type
+7m illumina: Gingeras: Transcript low copy can be a std feature in one cell but can change by 5 orders of magnitude in another cell type #AGBT
+8m ElementoLab: #AGBT Tom Gingeras: low copy number transcripts tend to be nuclear, but in specific compartments
+8m bioinfosm: if one were to spread out all the sessions (partying being the major part) at #AGBT to sequential events; it would be a month long affair!
+10m genome_gov: Now up - Geoffrey Smith: "The Miseq DNA Sequencing Platform and Application in Clinical Microbiology" #AGBT
+11m dgaalaas: Geoffrey Smith of ILMN talking MiSeq in clinical microbiology #AGBT
+11m ElementoLab: #AGBT Geoffrey Smith: using the Miseq for clinical microbiology applications
+12m dgaalaas: Uh-oh...just got the no-tweet disclaimer for this talk #AGBT
+15m NGSman: @dgaalaas #agbt There's always one
+15m bffo: GS from Illumina "under review, no tweet" is a very lame excuse that is becoming very bogus ... #badexcuse #verylame #agbt
+17m froggleston: GS: Can short turnaround time sequencing be applied to a clinical setting? I have sneaky feeling this will be a leading question. #agbt
+20m irondiet: OpGen Demonstrates Whole Human Chromosome Mapping Capabilities at 2012 AGBT ...: OpGen, Inc., a whole-genome ana... http://t.co/pB9ka95g
+22m mbcf: #AGBT finch and Duck. Tod lunches with RD http://t.co/Kiozg8Rq
+24m stuka: I'm feeling lonely w/ everyone @ retreats or #AGBT, so I think I throw a few hundred thousands jobs into the cluster so I can get some email
+27m Personal_RX_WI: #AGBT @illumina MiSeqused to track antibiotic resistant bacteria in hospitals. Clinical 2 day turn around time.
+28m CRIgenomics: #AGBT: Some lovely examples of the impact of sequencing on clincal microbiology by Geoff Smith. Look out for publications coming soon...
+31m girlscientist: Eric Green asks Geoffrey Smith of Illumina the $1M question: where do you actually see MiSeq's being deployed? A: in clinics, we hope #AGBT
+33m bmahersciwriter: RT @girlscientist: Eric Green asks Illumina the $1M question: where do you actually see MiSeq's being deployed? A: in clinics, we hope #AGBT
+36m girlscientist: Judging by the questions, people are intensely curious about issues surrounding use of MiSeq in clinics. Validation? Translation? #AGBT
+40m CompleteGenomic: Heres another chance to see Jason Laramie presenting on family-based studies, today at the #AGBT Complete Suite at 1:00 pm
+44m assemblathon: RT @djschlesinger: Schwartz: De novo assembly capabilities has not kept pace with increasing volumes of sequencing data #AGBT
+48m EpicentreBio: MT @girlscientist: EG asks GS of Illumina the $1M question: where do you actually see MiSeq's being deployed? A: in clinics, we hope #AGBT
+50m ScienceAdvBoard: RT @illumina: Myers: Correlation of methylation and gene expression depends on location of CpG #AGBT
+59m djschlesinger: Joseph Boland from NCI up next to discuss exome sequencing on the Ion PGM #AGBT

11 - 12pm EST
+0m DNASTARInc: Are you at #AGBT? Come see us at our poster featuring our #GWAS workflow today from 4:30-6:00 pm
http://t.co/7Bx7e75b
+4m EdgeBio: #AGBT Joe Boland from NCI talking about exome sequencing on the ion torrent
+4m girlscientist: Strap in for the lightning round on new technologies #AGBT Starting off with Ion Torrent and the PGM.
+4m nanopore: Wow, busy in here #AGBT
+5m deannachurch: Joseph Boland up after the break- talking about exome sequencing using the PGM. #AGBT
+6m dgaalaas: Welcome back to #agbt . Joe Boland talking about generating an Exome in a day on the PGM
+6m ElementoLab: #AGBT Joseph Boland: exome sequencing on the PGM: exome in a day
+7m deannachurch: JB: now routinely getting 250bp reads- up from 100bp reads in Q1. "Length does matter" #AGBT
+7m Massgenomics: Next up: Joseph Boland (NCI) on PGM exomes, Richard Roberts (NE bioLabs) on single-mol seq, Clive Brown of Oxford Nanopore! #AGBT
+7m omespeak: Second session of morning underway, most anticipated probably the last one from Clive Owen Of Oxford Nano. #AGBT
+8m iontorrent: within the span of one year with PGM: went from simply looking at barcode diversity to now, exome sequencing -Joe Boland #AGBT
+9m deannachurch: JB: Every 6-8 days producing 24-36 exomes on HiSeq but not a lot of flexibility in getting new samples in pipeline. PGM provides flex. #AGBT
+9m omespeak: Also, final plug for this blog-post on nanopore technology till it becomes irrelevant in about an hour: http://t.co/v331AKmW #AGBT
+9m djschlesinger: Boland: lab produces 24-36 exomes every 6-8 days #AGBT
+9m girlscientist: Boland singing praises of PGM for, among other things, meeting reviewer requests in two weeks. Hmmm. #AGBT
+9m finchtalk: #AGBT Joe Boland: exome in a day on the ion pgm. Uses, data fast for paper resubmission, impending site visits, fill pedigree gaps, backup
+9m dgaalaas: Exomes on a PGM gives Joe flexibility - main platform is HiSeq2000 #agbt
+10m EdgeBio: #AGBT Boland: first PGM arrived in Jan '11, first exome data generated by Nov
+10m dgaalaas: Joe currently generating an Exome on PGM via sequence across4-5 chips #agbt
+11m omespeak: Gah, bad typo ......meant Clive Brown of Oxford of course, not Owen. #AGBT
+14m EdgeBio: RT @dgaalaas: Joe currently generating an Exome on PGM via sequence across4-5 chips #agbt
+15m froggleston: @omespeak although I now look foolish as I can't seem to spell 'agbt'.
+15m AdamKraut: NCI showing impressive evolution of the PGM over the past year. Towards 'Exome in a Day' #AGBT
+15m EdgeBio: #AGBT Joe Boland: found that 5Gb/exome was the sweet spot
+16m omespeak: Second session of morning underway, most anticipated probably the last one from Clive Brown Of Oxford Nano. #AGBT
+16m Massgenomics: Joseph Boland on IonTorrent PGM "Exome in a Day". Sure, if you ignore the fact that hybridization takes 3 days. #AGBT.
+16m NEBiolabs: Rich Roberts "Characterization of DNA Methyltransferase Specificities Using Single-Molecule, Real-Time DNA Sequencing" NOW! #AGBT
+18m girlscientist: Boland and lab generated "exome in a day" after 5 days sample prep, if I read slide correctly. #AGBT
+18m bioinfosm: #agbt Boland's talk - am not sure if he defined the 'target region' that is referred in the talk
+19m Copenhagenomics: My guess: Most people at #AGBT are waiting for Oxford Nanopore's presentation... Are they going to present a game changer?
+19m ElementoLab: #AGBT Joseph Boland: can do exome in a day but with 6 machines
+20m CRIgenomics: The #ONT website is closed for maintenance, expect it to reopen just after Clive Browns talk later this morning at #AGBT
+20m omespeak: RT @CRIgenomics: The #ONT website is closed for maintenance, expect it to reopen just after Clive Browns talk later this morning at #AGBT
+22m girlscientist: Boland final conclusion: he needs to go back to school for bioinformatics. Don't we all? #AGBT
+23m salisburymw: How fast is tech advancing? J Boland: "This time next year this is going to seem prehistoric.". #agbt
+24m genome_gov: Up now: Rich Roberts NEB "Characterization of DNA Methyltransferase Specificities using Single-Molecule, Real-time DNA Sequencing" #AGBT
+24m froggleston: Borland: Interesting exome-in-a-day PGM talk. Were the pictures of kids the current staff mugshots? Child labour. Tut tut. #agbt
+24m deannachurch: Rich Roberts is up next talking about DNA methyltransferase specificities using single molecule sequencing. #AGBT
+25m girlscientist: Rich Roberts plays to crowd by asking if some of money being spent on DNA sequencing could instead be used figuring out what genes do. #AGBT
+25m djschlesinger: Richard Roberts from NEB talking about characterizing of DNA methyltransferase specificities using a PacBio #AGBT
+25m omespeak: Applause for Richard Roberts for saying fraction of money spent on sequencing should be spent on biology. #AGBT
+25m chrisfall: RT @GenomicsIo RT @girlscientist: Boland and lab generated "exome in a day" after 5 days sample prep, if I read slide correctly. #AGBT
+25m GenomeBiology: Spend some of the money used for sequencing genomes on finding out what genomes actually do. Richard roberts (NEB) #agbt
+25m deannachurch: RR: There is a lot of money being spent on sequencing- it would be nice to see some of that spent on understanding what genes do. #AGBT
+26m froggleston: Roberts: Lots of money spent on sequencing, and would be good to see the same amount spent on gene function studies. Cue clapping. #agbt
+26m Massgenomics: Richard Roberts hits the theme of the meeting: it would be nice to start spending some money figuring out what genes do. #AGBT
+26m djschlesinger: Roberts: a lot of money being spent on sequencing, what about money being spent on determining what it all means biologically? #AGBT
+27m ElementoLab: #AGBT Richard Roberts: we don't know the specificities of DNA methyltransferases (unlike for restriction enzymes)
+27m girlscientist: Can report that this is the first slide talk at #AGBT or perhaps ever I've seen with slides in vertical dimension. Am fixated on it. #AGBT
+27m Copenhagenomics: Richard Roberts: "Can we use a fraction of the $$$ we spend on sequencing, to actually find out what the genes are doing?" #applause #AGBT
+27m CRIgenomics: Rich Roberts NEB at #AGBT: can we spend a small fraction of the $$$ going into NGS on finding out what the genes do! Function rocks!
+30m Massgenomics: Was it fair when I asked about sensitivity of PGM exome-in-a-day sequencing? I worry that they found 10 of 16 cancer variants (62.5%). #AGBT
+31m ElementoLab: #AGBT Richard Roberts: use PacBio to show that EcoKDAM also recognizes also GATC sites, eg GACC
+32m girlscientist: #AGBT RT @jimcollins: @girlscientist I for one welcome our new exome overlords
+32m ElementoLab: #AGBT Richard Roberts: (I meant non-GATC sites) ... non-GATC sites might be due to high over-expression of methyltransferse
+33m Symbionticism: Rich Roberta of NEB shows PacBio detects methylated Cs due to slower sequencing through these bps in comparison to normal bps. Cool. #AGBT
+34m obahcall: New technologies session at #AGBT Joseph Boland just spoke on exome seq in a day (plus sample prep days) using PGM
+34m ElementoLab: #AGBT Richard Roberts: used PacBio SMRT seq to identify DNA motifs methylated by methyltransferases
+35m EdgeBio: A special thank you for all of our new followers! Stay tuned for more EdgeBio at #AGBT updates #FF
+35m drgitlin: @girlscientist when does AGBT registration usually happen? Need to make sure I'm approved to go beforehand.
+35m illumina: Richard Roberts, NEB, Characterization of DNA methyltrasferases specifcity using singe-iolecule real-time DNA sequencing #AGBT
+36m obahcall: Rich Roberts on char DNA methyltransferase specifities using PacBio #AGBT
+36m finchtalk: #AGBT Richard Roberts: e.coli methylases. Methylase free strains, plasmids, restriction enzymes, and pacbio to see specific target sites.
+38m CRIgenomics: How many labs name instruments after Star Wars characters? #AGBT Jo Boland has Yoda, Vader, Boba, etc. We have R2D2, et al...what about you?
+38m froggleston: Roberts: Lovely slides. No text. Easy to follow and back up his talk. Portrait a bit weird though. #refreshingchange #agbt
+39m ElementoLab: #AGBT Richard Roberts: found a methyltransferase that can methylate almost all As in the E coli genome ! it's M.ecoGI
+39m liveinsymbiosis: @Symbionticism That's cool. How much slower? #AGBT
+39m archivist84: So @nanopore are up soon at #AGBT
+39m girlscientist: Roberts: turns out E. coli can live with most of its genomic adenines methylated, as revealed by PacBio methylation sequencing. #AGBT
+41m DailyScan: Sequencing Takes a Clinical Bent at #AGBT http://t.co/k6QbldL2
+42m omespeak: 'ome' alert: Methylome. #AGBT
+42m froggleston: Roberts: Desire to see production of whole genome with accompanying methylation pattern as standard #agbt
+42m AdamKraut: Clive Brown from @nanopore up next. Wait for it. #AGBT
+42m RainDanceTech: Join us today at the RainDance #AGBT Workshop; 3:15pm in the Island Ballroom. Andy Watson will be discussing recent customer publications.
+43m djschlesinger: Clive Brown from Oxford Nanopore up next, he's been pacing back and forth for the past 10 minutes. Anticipations are high. #AGBT
+44m deannachurch: Clive Brown from ONT up next- standing room only in here. #AGBT #anticipation
+44m Copenhagenomics: Clive Brown from Oxford @Nanopore coming up now at #AGBT - perhaps the most anticipated presentation this year... Game changer coming up?
+44m EdgeBio: #AGBT much anticipated talk from Oxford Nanopore up next
+44m obahcall: More applications discussed by Eric Schadt in session tonight (thats after @girlscientist talk) #AGBT
+44m CRIgenomics: #ONT #AGBT...here it comes.
+44m finchtalk: #AGBT next up Clive Brown from Oxford Nanopore, a highly anticipated presentation. BTW, HDF is at the core of their data management.
+45m drgitlin: With the tweets and the fact that @nanopore's website is down, I'm getting the feeling that #AGBT is channeling Steve Jobs and Apple today.
+45m omespeak: Well, here it is. #nanopore #agbt
+46m dgaalaas: Clive Brown from Oxford Nanopore on deck here #agbt
+46m obahcall: Clive Brown Oxford Nanopore talk starting now at #AGBT
+46m deannachurch: CB: 2 hour talk in 17 minutes. #AGBT #go
+46m dgaalaas: Currently using protein nanopores #agbt
+46m ElementoLab: #AGBT Clive Brown: Nanopore sequencing
+46m omespeak: I will be mostly taking notes, so not much tweeting here. Will do summary later. #agbt
+46m t_basi: Breath baited. RT @omespeak: Well, here it is. #nanopore #agbt
+46m Symbionticism: Next up clive brown on oxford nanopore sequencing. #AGBT
+47m djschlesinger: Brown: this talk usually takes 2 hours, but I'll try to get it down to 17 minutes #AGBT
+47m nanopore: Nanopore sensing 101: current through nanopore, molecules interacting with nanopore generate characteristic disruptions, I/D molecule #AGBT
+47m Copenhagenomics: CB, @Nanopore "We have spent the last two years developing our own chemistry" #AGBT
+47m dgaalaas: Have developed their own proprietary chemistry - 400 bases per second #agbt
+48m djschlesinger: Brown: giving s brief overview of Nanopores and strand sequencing, nothing new yet #AGBT
+48m deannachurch: CB: attach a hairpin to the strand so you can sequence one strand then flip around and sequence the other. #AGBT
+48m CRIgenomics: #ONT Hungry Hippos at #AGBT
+48m dgaalaas: Enzyme feeds sense &antisense through custom Nanopore #agbt
+48m graveley: Holy cow - Oxford Nanopore @dgaalaas: Have developed their own proprietary chemistry - 400 bases per second #agbt
+48m UK_Biomek: No pressure on you @nanopore for #AGBT , but it seems like the whole of bio-twitter is expecting something earth-shattering...
+48m froggleston: Brown: Hungry Hippos. I knew it! #agbt
+49m dgaalaas: No fixed runtime - just refresh solution #agbt
+49m djschlesinger: Brown: no attachment of DNA, all free flowing, sequence read in real time, only requirement for sample prep is dsDNA have 5' overhang #AGBT
+50m dgaalaas: Sensor can handle 1000bps #agbt
+50m Copenhagenomics: CB, @Nanopore "ASIC is the core. Can do 1000bps. Cameras can't do that." #AGBT
+50m dgaalaas: Can do parallel sensing - multiplexing using an ASIC #agbt
+51m djschlesinger: Brown: alternative sample prep methods have been tried #AGBT
+51m finchtalk: #AGBT CB uses enzymes to pull DNA through pores, or block movement. over 160 tested. Measurement onASIC (app spec. integrated circuit)
+51m ElementoLab: #AGBT Clive Brown: only dsDNA with 5' overhang is needed
+51m AdamKraut: @nanopore uses ASICs, no fixed run time, capable of 1000bps #AGBT
+51m djschlesinger: Brown: membranes in sensor chip are very stable #AGBT
+52m illumina: Brown: ONT #AGBT Application Specific Integrated circuits instead of cameras
+52m notSoJunkDNA: Can do, or actually DO? @dgaalaas: Have developed their own proprietary chemistry - 400 bases per second #agbt
+52m girlscientist: Brown of Oxford Nanopore at light speed through their technology. Many comparative asides of how superior to current methods. #AGBT
+53m nanopore: Oxford Nanopore has engineered+screened >1,000 diff pores and >160 enzymes. Using unpublished proprietary system, continue to iterate #AGBT
+54m NowgenCentre: Currently reading tweets about Oxford @Nanopore presentation at #AGBT
+54m nanopore: Nanopore movement scheme: dsDNA+enzyme engages with pore, enzyme unzips DNA so sense and antisense in 1 pass many fragments (hairpin) #AGBT
+54m NGSman: @ElementoLab #agbt it was actually 5 :)
+54m froggleston: K-mers travelling through pore blockades provide basis for base calling. Neat. #agbt
+54m djschlesinger: Brown: sequenced PhiX genome as full genome read - 5kb and 10kb reads #AGBT - wild!
+54m deannachurch: CB: Sequenced PhiX: whole 5kb genome as single fragments, 4% error rate with deletions dominating. Read length = fragment size. #AGBT
+54m nanopore: Nanopore movement scheme: after strand 1 completes, a new DNA+enzyme strand reloads, and repeat #AGBT
+54m girlscientist: Brown: "so we did a genome" = PhiX. See about 4% raw read error, mostly deletions due to base caller. #AGBT
+54m Copenhagenomics: CB, @Nanopore "4% raw read error. Mostly deletions." #AGBT
+54m nanopore: Sample prep: only requirement dsDNA with overhang. No amplification. Variety of app-specific preps available. #AGBT
+54m dgaalaas: PhiX genome as single fragments, 5kb and 10 kb dsDNA reads! #agbt
+54m illumina: Brown: ONT #AGBT Lots of enzymes and pores tried
+55m nanopore: ASIC at the heart of the technology. Low noise, high freq chip designed to measure 1,000 bases per second on '000s arrays #AGBT
+55m djschlesinger: Brown: 4% raw read error, primarily deletions #AGBT
+55m ElementoLab: #AGBT Clive Brown: seq whole phix as 1 fragment .. 5kb or 10kb reads ... 4% raw error rate mostly deletions
+55m nanopore: Sensor chip: arrayed wells each with electrode measuring single nanopore experiment. #AGBT
+55m dgaalaas: No degradation of signal over time #agbt
+55m deannachurch: CB: Not limited by fidelity of system- limited by sample fragmentation. No diff between base 1 and base 40,000. #AGBT
+55m djschlesinger: Brown: no degradation of signal over time base 10 same as base 40,000 #AGBT
+55m Copenhagenomics: CB, @Nanopore "We did a 48kb run. Quality consistent across reads - no difference between first and 40000th read!" #AGBT
+55m nanopore: Base calling: overlapping kmers cause characteristic current blocks, Viterbi path. Done in real time on node #AGBT
+55m finchtalk: #AGBT CB reading full length sequences, 10 kb for phiX. And recently the lambda genome. No signal degradation. Reads words not bases.
+56m EdgeBio: #AGBT Clive Brown: if genome is small enough it can be fully sequenced as a single fragment
+56m girlscientist: Brown: no degradation of signal over time. Principal cause of error known, engineering barrel of pores to compensate. #AGBT
+56m nanopore: 5kb genome sequenced sense and antisense (with hairpin) in single 10kb run. Further examples at tens of kbs #AGBT
+56m dgaalaas: Improvements - removal of variance needed #agbt
+56m EdgeBio: RT @dgaalaas: No degradation of signal over time #agbt
+56m froggleston: Brown: Oscillating k-mers produce majority of error. Fixable. Dips in quality with erroneous bases. #agbt
+57m Symbionticism: Oxford nanopore has sequenced largest reads ever - up to 40 kb but with 4% error. They will surpass them said clivebrown. Exciting at #AGBT.
+57m EdgeBio: RT @dgaalaas: Improvements - removal of variance needed #agbt
+57m djschlesinger: Brown: can directly read RNA, no cDNA required #AGBT
+57m ElementoLab: #AGBT Clive Brown: RNA can be DIRECTLY read using nanopores - no cDNA
+57m girlscientist: Brown: "we had a quick look at RNA" can be read directly with no need for cDNA, data look same as DNA. #AGBT
+57m deannachurch: CB: RNA can be read directly using nanopores- no need to make cDNA. #AGBT
+58m Copenhagenomics: CB, @Nanopore "RNA can be read directly. No cDNA required. This is an application we will put out at a later stage." #AGBT
+58m notSoJunkDNA: I want one! @ElementoLab: #AGBT Clive Brown: RNA can be DIRECTLY read using nanopores - no cDNA
+58m dgaalaas: Should be able to process RNA - development of base calling for RNA on hold #agbt
+58m bioinfosm: #agbt nanopore talk "the enzyme ticks after each nucleotide to overcome homopolymer issue"!! long reads, max 41kb single molecule read
+58m graveley: Wow!! @djschlesinger: Brown: can directly read RNA, no cDNA required #AGBT - looks like game changer!
+58m girlscientist: Brown: excellent shoutout to Monty Python rabbit. #AGBT
+58m Symbionticism: Wow. Oxford nanopore will sequence RNA too at same efficiency as DNA. more to come. #AGBT
+58m westr: RT @DailyScan: Sequencing Takes a Clinical Bent at #AGBT http://t.co/JLFOIksm #pm101
+59m ElementoLab: #AGBT Clive Brown: epigenetic modifications can be detected using nanopores too eg methylation
+59m neilhall_uk: Want! #agbt
+59m nanopore: Measuring direct from blood: new robust polymer membranes resistant to complex sample. #AGBT
+59m djschlesinger: Brown: sequenced rabbit DNA extracted from blood, no sample prep at all!!! #AGBT
+59m dgaalaas: Can sequence directly froml blood - no sample prep necessary... #agbt
+59m Copenhagenomics: CB, @Nanopore "DNA directly from blood. No sample prep required." #AGBT
+59m deannachurch: CB: 0 sample prep- directly sequenced rabbit blood and sewage water. #AGBT #supercool

12 - 1pm EST
+0m fluidigm: For Targeted Resequencing and Single Cell Studies swing by Suite 285 at #AGBT, let us show you what we got!>o
+0m girlscientist: Brown: "Just one more thing..." new product with single use disposable sequencer #AGBT
+0m illumina: Brown: Sequencing, RNA and epigenetic mods with ONT #AGBT
+0m Symbionticism: Nanopore will sequence directly from blood and sewage water. #blownaway #AGBT
+0m deannachurch: CB: MinION: usb sized DNA sequencer- single use disposable sequencer. #AGBT
+0m nanopore: Hardware: a single GridION node can be used alone as a desktop or lots clustered. Each node analyses data locally in real time. #AGBT
+0m cwduffy_lshtm: So going by the #AGBT tweets @nanopore may have completely changed the face of sequencing. 40kb+ reads! Can't wait to see some publications.
+0m AdamKraut: And one more thing MiniION. USB key size sequencer that plugs into your laptop. Same data as GridION #AGBT
+0m djschlesinger: Brown: one more thing: MiniION portable Nanopore sequencer, plugs right into USB port of your laptop #AGBT
+0m dgaalaas: Going to launch MinION later this year, a USB disposable sequencer. wow! Plug it directly into your laptop.... #agbt
+0m Massgenomics: Oxford MinION USB-sized disposable DNA sequencer, 150 Mbp per hour for 6 hours.. YHGTBFKM! #AGBT.
+0m liveinsymbiosis: @Symbionticism That's amazing!!! Will it be competitive cost/bp? #AGBT
+0m nanopore: And now for something completely different #AGBT
+1m girlscientist: Brown: competitive with current desktop prices at launch, Tolerates body fluids and plant extracts. #AGBT There's your headline, people.
+1m omespeak: Plug and play sequencer from Oxford Nano. #agbt
+1m nanopore: Introducing the MinION, a nanopore sequencer the size of a USB stick. Works just like GridION, but smaller #AGBT
+1m girlscientist: Brown: new sequencer called MinION, powered from USB on your laptop. #AGBT
+1m ElementoLab: #AGBT Clive Brown: MinION sequencer basically looks like a USB key
+1m djschlesinger: Brown: sequencer will be USB powered and disposable #AGBT
+1m girlscientist: Brown: MinION app-driven and open. "lets any bench scientist put their arms around process" of sequencing. #AGBT
+1m Copenhagenomics: CB, @Nanopore "We're launching a USB sized disposable sequencer later this year: 'MinION' - 150MB/hr, runtime 6 hrs." <-- Amazing! #AGBT
+2m rowan_UK: Following @nanopore tweets from #AGBT. Smokin performance reported by Clive Brown
+2m AdamKraut: You can even yank the USB sequencer out, it pauses, re-insert and it continues sequencing #AGBT
+2m finchtalk: #AGBT CB sequenced rabbit blood directly, sewage with minimal prep. MiniION, a USB sequencer. Just need a laptop and software.
+2m drgitlin: Game changer? Wow. RT @nanopore: Measuring direct from blood: new robust polymer membranes resistant to complex sample. #AGBT
+2m pathogenomenick: Too many amazing @nanopore tweets to retweet them all. Follow #AGBT or go to http://t.co/KUVmX75N to see them all.
+2m froggleston: Holy crap. Minion. A USB sized sequencer with real time informatics. This changes the game. I'm grinning. Electrochemistry issues tho #agbt
+2m djschlesinger: Brown: MiniION launched later this year, 5-25 Gb per day, chip will last ~6 hours #AGBT
+2m notSoJunkDNA: MinION sounds like "mignon" in French, which means cute! @nanopore: Introducing the MinION, a nanopore sequencer #AGBT
+2m 613SportsChick: Collective gasp at #AGBT as Brown announces the MinIon.
+2m nanopore: GridION node 2012: 2,000 nanopore experiments at a time, in 2013 to 8,000. Node designed for tens of Gb/24 hours, no fixed run time #AGBT
+3m bkmacy: Nanopore discusses new MinION USB 3.0 based personal DNA/RNA sequencer later this year, #AGBT
+3m NGSman: #agbt miniIon....outrageous!
+3m omespeak: If the machines perform as promised, The Oxford Nano announcements are indeed quite game changing. #agbt
+3m ElementoLab: #AGBT Clive Brown: MinION can seq 1-5 Gb/day
+3m djschlesinger: Brown: future improvements will extend that to over 600Gb per day #AGBT
+3m archivist84: MiniION from @nanopore - in the conference goody bag next year? #AGBT
+3m drgitlin: OMFG RT @nanopore: Introducing the MinION, a nanopore sequencer the size of a USB stick. Works just like GridION, but smaller #AGBT
+3m girlscientist: Brown: price list. MinION about $1000/Gb, will retail at $500-$1000. #AGBT
+3m rowan_UK: Buy disk stocks MT @nanopore Introducing the MinION, a nanopore sequencer the size of a USB stick. Works like GridION, but smaller #AGBT
+3m AdamKraut: MiniION will cost between $500-$1000. Lower cost per Gb with GridION #AGBT
+4m liveinsymbiosis: @djschlesinger Is the ~6hr a limitation/exhaustion of the chemistry? #AGBT
+4m nanopore: New pricing schemes: no more box/instr price.Think cellphone pricing - packages. Price per base competitive at launch with other techs #AGBT
+4m mbeisen: i'm not at #AGBT but @nanopore USB sequencer sounds totally badass
+4m Symbionticism: NANOPORE will introduce usb- drive sequencer called minion. Benchtop sequ. in laptop. 150mb per hr if i heard that right. $1000. #AGBT
+4m Massgenomics: The throw-away USB sequencer, second half of this year, will cost $500-1000 per use, supposedly. #AGBT.
+4m DailyScan: Truly! #AGBT MT @pathogenomenick Too many amazing @nanopore tweets to retweet them all.
+4m AdamKraut: Always knew @nanopore was the dark horse. Clive just killed it! #AGBT
+4m Copenhagenomics: CB, @Nanopore "Nanopore sequencing is high-throughput. MinION $1000/Gb. GridION2K $25-40/Gb. GridION8K $10/Gb" #AGBT
+4m girlscientist: Brown: future = GridION cluster. <$10/Gb by hooking up cluster together. #AGBT Eric Green "I can't imagine there are any questions."
+4m djschlesinger: MiniION - holy crap!!!!!! Amazing!!!! #AGBT
+4m AdamKraut: First question: "When can I buy one?" #AGBT
+4m djschlesinger: MiniION available second half of this year #AGBT
+5m ElementoLab: #AGBT Clive Brown: MinION to be released second half of this year
+5m CRIgenomics: gridion and MinIon coming mid 2012 #AGBT #ONT really rocks...
+5m obahcall: Will be available second half of this year... #AGBT
+5m froggleston: GridION and MinION available Q3 2012. Want. #agbt
+5m Symbionticism: Will be released in 2nd half of year. #AGBT
+5m bkmacy: Nanopore MinION expected to cost $500-1000 / experiment, #AGBT
+5m aaron_best: RT @pathogenomenick: Too many amazing @nanopore tweets to retweet them all. Follow #AGBT or go to http://t.co/9XtYwi30 to see them all.
+5m Copenhagenomics: CB, @Nanopore "MinION and GridION2K will be launched in the second half of 2012." <-- Q4 perhaps? #AGBT
+6m girlscientist: Eric Green asks if MinION will work on Mac and PC -- Brown says he is Mac user to applause from room. #AGBT
+6m BARCLABS: AGBT: Oxford Nanopore to Begin Selling Two Low-Cost DNA Strand Sequencing Instruments this Year:
read more

http://t.co/P3Y9TDFz
+6m Symbionticism: Yay! Minion will work on your macs. #AGBT
+6m pathogenomenick: The full Oxford Nanopore press release up here http://t.co/JnJHjsD4 #AGBT
+6m Personal_RX_WI: #AGBT Oxford Nanopore MinION USB sized disposable sequencer. Available sencond half of 2012.150Mb/hour
+7m genomigence: InSequence: AGBT: Oxford Nanopore to Begin Selling Two Low-Cost DNA Strand Sequencing Instruments this Year:
r... http://t.co/NbamPTje
+7m InSequence: AGBT: Oxford Nanopore to Begin Selling Two Low-Cost DNA Strand Sequencing Instruments this Year:
read more

http://t.co/RDy3CMMs
+7m archivist84: http://t.co/Pz7eFAub #OGT website is back up! #AGBT
+7m Copenhagenomics: I do think @nanopore presented a potential game changer today: The MinION brings something totally new, with disposable sequencers! #AGBT
+8m AdamKraut: Small mob forming around Clive now #AGBT
+8m pathogenomenick: Oxford Nanopore megaton announcement: Why do you need a machine? exclusive interview on my blog! http://t.co/tJxQit9T #AGBT
+9m rnomics: RT @notSoJunkDNA: I want one! @ElementoLab: #AGBT Clive Brown: #RNA can be DIRECTLY read... http://t.co/MPjnke5h #rnomics-bioscience @MyEN
+9m MoleculaBiology: Oxford Nanopore minIon announced at AGBT. Unbelievable. The revolution is here for single molecule sequencing.
+9m CrapBio: SO what i am hearing is that Oxford Nanopore have taken all the fun out of sequencing. #AGBT
+10m Symbionticism: MiniION will make the tricorder a reality:-) awesomeness at #AGBT
+10m rnomics: RT @deannachurch: CB: #RNA can be read directly using nanopores- no need to make cDNA. #AGBT http://t.co/5dNOOxYt #phylogenomics-gen @MyEN
+10m westr: RT @Personal_RX_WI: #AGBT Oxford Nanopore MinION USB sized disposable sequencer. Available sencond half of 2012.150Mb/hour #pm101
+11m ChaunceyGrattan: My head is about to explode. #RNAtoo RT: @pathogenomenick The full Oxford Nanopore press release up here http://t.co/Jp4DCgqP #AGBT
+11m liveinsymbiosis: Welcome to the new age of sequencing tec. AGBT: Oxford Nanopore to Begin Selling Low-Cost DNA Sequencing http://t.co/nsXrplry @AddThis #AGBT
+11m InSequence: Details on ONT's GridIon and disposable nanopore sequencer, MinIon: http://t.co/WwPKPXQ5 #AGBT
+12m UK_Biomek: Woah RT @nanopore: Introducing the MinION, a nanopore sequencer the size of a USB stick. Works just like GridION, but smaller #AGBT
+13m westr: RT @InSequence: Details on ONT's GridIon and disposable nanopore sequencer, MinIon: http://t.co/pcJC5Wtu #AGBT #pm101
+13m mcaccamo: CB: 48KB lambda genome in one read #agbt
+13m ezequiel_coelho: #AGBT Does anyone know the price for the grid ION from oxford nanopore?
+14m avsmith: Those Ion Torrent tweets suddenly got much less interesting. Drooling over Oxford Nanopore reveal at #agbt.
+14m liveinsymbiosis: #AGBT this will be great for bringing sequencing tech to the classroom.
+16m sumansen2011: Affordable and accessible DNA sequencing, a big step in personalized medicine http://t.co/X3I345iJ via @AddThis
+16m notSoJunkDNA: So, it's for real? DNA sequencer the size of a USB key: http://t.co/VSOyO4WD #AGBT (via @nanopore )
+17m bethasullivan: wow, so exciting! MT @girlscientist Brown (of Oxford Nanopore): new sequencer called MinION, powered from USB on your laptop. #AGBT
+18m ribozyme: Agreed! RT @bffo: GS from Illumina "under review, no tweet" is a very lame excuse that is becoming very bogus .. #badexcuse #verylame #agbt
+20m omespeak: So guess what the lunch time discussions will be at #AGBT?
+20m assemblathon: RT @pathogenomenick: Too many amazing @nanopore tweets to retweet them all. Follow #AGBT or go to http://t.co/pCPsTBaw to see them all.
+20m GenomeWeb: Big stories from this year's #AGBT? It will be hard to beat Oxford Nanopore's announcement of the disposable MinIon http://t.co/P6eVIKjz
+21m rowan_UK: True! RT @avsmith
Those Ion Torrent tweets suddenly got much less interesting. Drooling over Oxford Nanopore reveal at #agbt.
+22m assemblathon: RT @djschlesinger: Brown: sequenced rabbit DNA extracted from blood, no sample prep at all!!! #AGBT
+22m assemblathon: RT @deannachurch: CB: MinION: usb sized DNA sequencer- single use disposable sequencer. #AGBT
+22m timjph: New sequencers announced at #AGBT by Oxford Nanopore; one USB sized @pathogenomenick: full press release here http://t.co/fYNWJYEf #AGBT
+23m NextBio: Exciting news! RT @nanopore: Introducing the MinION, nanopore sequencer the size of a USB stick. Works just like GridION, but smaller #AGBT
+23m kbradnam: A disposable sequencer is the perfect successor to years of throwaway sequencing data ;-) #AGBT
+24m assemblathon: RT @Massgenomics: Oxford MinION USB-sized disposable DNA sequencer, 150 Mbp per hour for 6 hours.. YHGTBFKM! #AGBT.
+24m assemblathon: RT @djschlesinger: Brown: MiniION launched later this year, 5-25 Gb per day, chip will last ~6 hours #AGBT
+25m assemblathon: RT @girlscientist: Brown: price list. MinION about $1000/Gb, will retail at $500-$1000. #AGBT
+26m jung_gt: For real? RT: @Massgenomics: Oxford MinION USB-sized disposable DNA sequencer, 150 Mbp per hour for 6 hours.. YHGTBFKM! #AGBT.
+26m nparmalee: I assume that all the geneticists I know are already following the #AGBT hashtag. If not, you should be. #FF
+26m morinryan: Keith Robison's blog post (Omics! Omics!) on Oxford Nanopore's big #AGBT reveal is now live: http://t.co/UQ7mHm2H
+28m rowan_UK: @zerojinx @nanopore Who do I have to mug to get a MinION? #AGBT
+28m illumina: Loman and Robison on #AGBT big news, the MiniIon http://t.co/EUYEpp1d and http://t.co/iGRNR6gq
+29m bioitworld: Full story on Oxford @nanopore stunning presentation from CTO Clive Brown at #AGBT http://t.co/VNvCxLQN
+29m fluidigm: LEARN about targeted resequencing on all major next-gen sequencing systems. Stop by Suite 285. #AGBT ^S
+29m archivist84: Too much excitement! Too many NGS slides to change next week! Off to the pub! #AGBT #OGT #nanopore
+29m sargoshoe: Is it weird that I'm excited about this? RT @omespeak: Well, here it is. #nanopore #agbt
+30m iontorrent: Join Us - Ion lounge at AGBT, Friday, 9:00 p.m.--2:00 a.m.: http://t.co/OExjUIVf via @youtube
+30m Copenhagenomics: RT @larsgt: Wouldn't want to be the guy running the PacBio workshop this afternoon... #AGBT
+30m westr: Thanks to all the tweeters at #AGBT for keeping the rest of us informed about sequencing & genomic breakthroughs!
+31m Jeylen: +1 Wow @pathogenomenick: Oxford Nanopore megaton announcement: Why do you need a machine? exclusive interview http://t.co/7TXozcQo #AGBT
+32m EpicentreBio: Keith Robison on Oxford Nanopore #AGBT announcements http://t.co/wh6Vvmp4
+32m AdamKraut: How far will the PacBio, Illumina, and Life stocks drop after that? #AGBT
+32m assemblathon: RT @pathogenomenick: The full Oxford Nanopore press release up here http://t.co/RzKT3s4f #AGBT
+33m nparmalee: #FF @girlscientist Chris Gunter tweeting from #AGBT This is what twitter is for.
+33m ijivanjee: @pathogenomenick Great interview, this technology blows my mind! #AGBT #ONT
+33m pathogenomenick: jeez I need a lie-down at this point #AGBT @nanopore
+34m ribozyme: Go Mac! RT @girlscientist: Eric Green asks if MinION will work on Mac and PC -- Brown says he is Mac user to applause from room. #AGBT
+35m LucigenCorp: Need libraries for NexGen Seq? Sample prep solutions you can use for better libraries! Posters at #AGBT:http://t.co/iUh67GSb
+35m kbradnam: RT @CrapBio: SO what i am hearing is that Oxford Nanopore have taken all the fun out of sequencing. #AGBT
+37m genetics_blog: Cool. Oxford Nanopore's tiny DNA sequencing instrument - (MinION) doubles as USB drive #AGBT http://t.co/NfsIUEsD http://t.co/AMNU6m3v
+39m SEQanswers: Vaporware is in the air at Marco Island. Oxford Nanopore owes me a thorough computer monitor cleaning. #AGBT
+40m leonidkruglyak: "Hard to compete against reductions in cost, labor and input DNA reqs, esp when data is many fold more useful." http://t.co/DT2WMfJH #AGBT
+40m djschlesinger: MiniION - haven's been this excited about a piece of technology since the original iPhone. #AGBT
+40m djschlesinger: MiniION - haven's been this excited about a piece of technology since the original iPhone. #AGBT
+40m ribozyme: This year at #AGBT: a USB stick giveaway with data from trios; next year a USB stick disposable sequencer giveaway - awesome!
+41m djschlesinger: MiniION - will Life sue over the use of the name "Ion"?? #AGBT
+41m kristen_hallam: Also, it's possible to sequence a human genome in 15 min. using @nanopore's GridION system. http://t.co/MZhO16lr #agbt
+41m leonidkruglyak: "would the processivity limits be reached at BAC scale? Whole bacterial chromosomes? Whole yeast chromosomes?" http://t.co/DT2WMfJH #AGBT
+42m genome_gov: Lots of great tweets from the Marco Island meeting! Interested? Follow the #AGBT hashtag
+42m CompleteGenomic: Today at #AGBT, catch customer Carlos Bustamante of Stanford U. present on population genetics at 2:55 pm in Islands Ballroom
+43m djschlesinger: MiniION - kind of wish the made the announcement on Saturday, the rest of the meeting won't be nearly as exciting #AGBT :p
+43m AdamKraut: People are literally on the phone with their brokers right now #AGBT
+43m drgitlin: If @nanopore's 4% error rate is uniform, does that mean it goes away with high enough coverage? #AGBT
+44m MicheleClamp: So the fancy new OxNano pocket sequencers I can finally rave about!!! Sequencing has finally broken free of the lab!! #AGBT
+45m t_basi: Too right! RT @AdamKraut: People are literally on the phone with their brokers right now #AGBT
+46m mrkrly: #FF #FollowFriday @nanopore - tweeting the future from #AGBT today!
+46m akmi3: MinIon, USB sequencer for $900 with 512 nanopores, 150mb/hour. Disruptive Innovation! http://t.co/wArJWkLV #DigitalHealth #personalmed #AGBT
+47m BioInfo: AGBT: Oxford Nanopore to Begin Selling Two Low-Cost DNA Strand Sequencing Instruments this Year | In Sequence | Sequencing | GenomeWeb http:
+51m BozelosP: @nanopore DNA sequencer with USB plug for $900 - 512 nanopores, 150mb/hour - Disruptive Innovation! http://t.co/vXOqpFcj #AGBT
+51m dud3k: Bets on Roche lowering its bidding price for $ILMN after today? #agbt Also, anyone short $PACB, $ILMN and $LIFE? =)
+51m cwduffy_lshtm: Is it wrong that I want a minION just to put on my key chain? #AGBT #livinginthefuture
+52m nlbigas: A disposable USB pocket-sized nanopore sequencer. http://t.co/zqvmzkZz #AGBT
+53m Gavin_Oliver: Attempting to live-blog Clive's talk was an unmitigated disaster. Too much info. In a good way. Good work @nanopore #AGBT
+55m wboonn: Thx! RT @PopperandCo: It's #FF - @Paul_Sonnier @wboonn @Doherty_pd @kenwalz @portablegenomic @DivaBiotech @westr @illumina @agbt @Forbes
+55m markgenome: #AGBT tweet stream lit up, and no wonder. @nanopore had an amazing announcement up its sleeve. http://t.co/udJ6IvO8
+56m nparmalee: Can I make #AGBT requests? Would love to "hear" Carlos Bustamante's talk, 2:55p today, Islands Ballroom.
+56m genetics_blog: If looking through the #AGBT tag is too daunting, @pathogenomenick is doing a fine job summarizing at http://t.co/CWWf79Yi. Thank you sir
+56m lexnederbragt: Congrats to @pathogenomenick for the scoop, interview with @nanopore #AGBT
+57m Copenhagenomics: Gotta love the duality of "MinION" - even the name is cool! :) #AGBT
+58m timjph: @nanopore parodies Apple! Eyeing the future value of universal DNA sequencing? RT @girlscientist: Brown: "Just one more thing..." #AGBT

1 - 2pm EST
+0m j_perkel: Oxford Nanopore details GridION and MinION systems at AGBT: http://t.co/8keFNrKJ
+0m BozelosP: Introduction to Nanopore Technology http://t.co/dpRlpoYE 4th generation DNA sequencing is here.. Great news for personal genome era #AGBT
+1m nakedideas: We just launched the new @nanopore website in a #Apple Keynote style whirlwind for #AGBT So exciting! Congrats to them! http://t.co/EbsX3CfF
+1m liu3zhen: This video helps understand the Wow Nanopore tech: http://t.co/0tOJygpv #AGBT
+2m djschlesinger: Correct spelling is MinION, not MiniION. I was so excited to tweet about it, I forgot to spellcheck #AGBT
+2m iontorrent: Come to the Ion Lounge tonight! Beer, food and science on the beach. Meet us at the bus. #agbt #ionbus http://t.co/gpXIbNV6 via @youtube
+3m GenomicsIo: MT @nlbigas: A disposable USB pocket-sized nanopore sequencer. http://t.co/PfKkmrmg #AGBT Mind-boggling, but incredibly exciting!
+4m notSoJunkDNA: Starting today, we'll never hear @illumina or @SOLiDSequencing referred to as "Next-Gen Seq" But I think they still have a future. #AGBT
+4m nakedideas: We just launched the new @nanopore website in a #Apple Keynote style whirlwind for #AGBT So exciting! Congrats to them! http://t.co/U516x2Uo
+5m dud3k: Wow, them markets don't snooze. $ILMN $LIFE $PACB all down 5-6% in last hour. #agbt
+7m leonidkruglyak: "We wander into the realms of sci-fi at this point." @pathogenomenick interview with @nanopore http://t.co/5A6wlkfc #AGBT
+8m omespeak: Most commonly overheard snippet at #AGBT right now: 'it is amazing, but we have to see it work in practice'. No prize for guessing 'it'.
+8m kristen_hallam: Shares of $LIFE, $ILMN falling after Oxford Nanopore unveils portable DNA sequencing device, GridION scalability. #agbt
+10m notSoJunkDNA: Amen @omespeak: Most commonly overheard snippet at #AGBT right now: 'it is amazing, but we have to see it work in practice'
+12m dud3k: errr correction, on yesterday's close. RT @dudek Wow, them markets don't snooze. $ILMN $LIFE $PACB all down 5-6% in last hour. #agbt
+13m leonidkruglyak: Great problem to have: "how will 100kb+ reads change your research?" http://t.co/5A6wlkfc #AGBT
+15m yokofakun: @Fuzzyraptor oui a buzz fort la confrence #AGBT : un squenceur de la taille d'une clef USB: see also: http://t.co/lBCg8WaG
+15m SEQanswers: Waiting to see the 48.5kb lambda read. @nanopore ?? #AGBT #makemeeatmywords
+15m _a_muse: Excited about the Oxford plug and sequence announcement. And I am not at #AGBT . That place will be buzzing right now with discussions.
+17m michaelhoffman: @leonidkruglyak The first problem is I'm going to have to clean up all this drool. (sorry) #agbt
+17m caddymob: minions and gridions! Gridion (not grid-ion) will forever be the title bestowed on most productive summer intern. Thx #AGBT
+18m khedkarsupriya: Sequencing technology reads bases in triplets and detects them using electrochemistry http://t.co/dXhCVl0P http://t.co/ssFjZtpZ #AGBT
+18m omespeak: Wondering when we shall see real world data from Oxford Nanopore? #AGBT
+24m ijivanjee: I am in a state of shock. #AGBT #ONT
+25m RickyConnolly: "@nanopore: Introducing the MinION, a nanopore sequencer the size of a USB stick. Works just like GridION, but smaller #AGBT" neat tech
+26m pedrobeltrao: @mndoci just a very small selection bias :).those not at the AGBT meeting probably wished they were
+28m j_perkel: .@Erika_Check at @NatureNews on today's Oxford Nanopore announcement at AGBT: http://t.co/Cml2zV63. // A human genome in 15 minutes?
+34m kbradnam: Reactions to @nanopore news. Biologists: cheer. Bioinformaticians: groan. System admins: weep. #AGBT
+36m omespeak: Hmmm...... a 'nanopore war'!!! http://t.co/7S3iWnPG #AGBT #ONT
+37m jung_gt: Indeed! RT: @liveinsymbiosis: #AGBT this will be great for bringing sequencing tech to the classroom.
+37m GenomeBiology: Oxford nanopore had everyone talking at lunch. Is the minION going to be the next next gen? Wow. #agbt
+37m SEQanswers: Realists: spit take. @kbradnam: Reactions to @nanopore news. Biologists: cheer. Bioinformaticians: groan. System admins: weep. #AGBT
+37m druvus: Oxford Nanopore Doesn't Disappoint http://t.co/Hbf2tI6r #sequencing #AGBT
+40m schip: RT Watch ILMN rebound on purchase of Oxford @dudek Wow, them markets don't snooze. $ILMN $LIFE $PACB all down. #agbt
+45m illumina: How did we get 4 whole human genomes on 1 USB drive? Find out at our talk in the Island Ballroom at 2:15p #AGBT
+45m dfjenkins3: Only after nerding out. RT @SEQanswers: Realists: spit take. @kbradnam: Reactions to @nanopore...Bioinformaticians: groan...#AGBT
+47m kbradnam: Every picture tells a story #AGBT http://t.co/trbX0XFX
+48m Matt_Francis: Hard act2follow RT @illumina How did we get 4 whole human genomes on 1 USB drive? Find out at our talk in the Island Ballroom at 2:15p #AGBT
+49m assemblathon: RT @genetics_blog: If looking through the #AGBT tag is too daunting, @pathogenomenick is doing a fine job summarizing http://t.co/vXy0mFRw
+49m DPMelters: RT @kbradnam Every picture tells a story #AGBT http://t.co/bsh3FiJI
+50m Matt_Francis: #AGBT so the nanopore + a laptop is almost a star trek tri-corder. #futureishere #wheresmyhoverboard
+52m girlscientist: Oh yeah? Well, our new R10K project gives free reagents! And it's sexy! And you can use a USB drive for it, kind of! Find out at 7:30. #AGBT
+55m Bobmorevc: Moore's law blown away @kbradnam: Reactions to @nanopore news. Biologists: cheer. Bioinformaticians: groan. System admins: weep. #AGBT
+58m kbradnam: You will now be able to tell your kids "I remember when USB thumb drives were only used to store files" #AGBT

2 - 3pm EST
+0m fluidigm: IT'S NOT TOO LATE!! Stop by and learn about eliminating the need for paired-end read sequencing. Suite 285. #AGBT ^S
+0m EquallyBalance: RT @GenomeBiology: Oxford nanopore had everyone talking at lunch. Is the minION going to be the next next gen? Wow. #agbt
WOW!
+1m illumina: Illumina Talk: From Sample to Answer: Fast and Accurate Genome Seq for the Clinic -- starts in 10 min, Island Ballroom #AGBT
+2m assemblathon: I wonder if tourism to Oxford, UK will see a little spike in the coming months? #AGBT
+2m BioMickWatson: I'm a bioinf not groaning! RT @kbradnam Reactions to @nanopore news. Biologists: cheer. Bioinformaticians: groan. System admins: weep. #AGBT
+4m lexnederbragt: I guess 100kb reads make the bioinformatics of whole genome assembly problem go away... #ONT #AGBT
+5m emmecola: NGS: News on Genomic Studies: Flash report: two new Next Generation Sequencers presented at AGBT http://t.co/vh3Qpd9l
+5m illumina: Come pick up a "Genome on a Stick" in the Illumina Lounge after Elliott Margulies' talk. 4 genomes, 1 USB drive. #AGBT http://t.co/KNW7EMzr
+11m BiotechMarketer: Looks like @nanopore stole the show with 15 min talk. Did all other platform cos waste their marketing budgets at #AGBT ?
+12m WeigelWorld: Sequencing on a USB stick? http://t.co/aZh8Z7f5
+13m folded: Someone should go and park an #ONT branded matchbox bus next to the #ionbus at #agbt.
+14m rob_carlson: Recalling that just two months ago several prominent biologists assured me #nanopore sequencing was fairy dust. Oops. #AGBT
+15m maureen_mack: hey @Personal_RX_WI apparently @illumina is giving away genomes on a stick #agbt don't miss it!
+16m omespeak: Afternoon sessions just starting. Will anyone actually listen? #AGBT #ONT
+23m ribozyme: I want sequencers on a stick! @maureen_mack, RT: @illumina is giving away genomes on a stick #agbt don't miss it! #AGBT
+25m CompleteGenomic: Find out more about our Cancer Sequencing Service from our own Srinka Ghosh, in the #AGBT Complete Suite at 4:15pm
+26m pathogenomenick: I've embedded all the nanopore videos on the blog here http://t.co/rhBpP9XS #AGBT
+27m rob_carlson: ILMN cheers storing genomes on a USB stick while Oxford #Nanopore was showing sequencing genomes WITH a USB stick? #AGBT #futureshock
+27m Personal_RX_WI: #AGBT @illumina HiSeq2500 DNA in to gene annotation in 60hr 40 min. Perfect for clinical whole genome sequencing.
+27m ribozyme: 4% error still needs deep cov; also mixtures (i.e. cancer), het calls also. RT @drgitlin: goes away with high enough coverage? #AGBT
+28m GarimaKushwaha: "Miniaturized, disposable USB DNA-sequencer" OH MY GOD!!! #amazing #AGBT
+32m omespeak: Market reacts to #ONT announcement at #AGBT RT @matthewherper: The Next DNA Disruptor? - Forbes http://t.co/ZCLKzhXi
+35m illumina: Marguiles: Data integration from multiple databases including medical risk data w/ variant calls using ISAAC to detect causal muts #AGBT
+36m illumina: Marguiles: Accelerated analysis, 7 hrs for total analysis workflow w/ iSAAC + GATK, one command operation #AGBT
+37m illumina: Marguiles: 120 Gb BAM reduced to just 6 Gb as CRAM file #AGBT
+37m westr: RT @pathogenomenick: I've embedded all the nanopore videos on the blog here http://t.co/6uD2uSzc #AGBT #pm101
+38m lykkebak: eller Video from #AGBT with Anja about #CPHx 2012 June 14-15 http://t.co/w5Vnp0kL
+42m leonidkruglyak: RT @pathogenomenick: I've embedded all the nanopore videos on the blog here http://t.co/C7CrCTMO #AGBT
+42m finchtalk: #AGBT Kevin Hrusovsky on where PerkinElmer is going, mentions that 4th leading cause of death: drug side effects.
+44m kbradnam: The @nanopore press release also reveals that the MinION syncs with iTunes, acts as a 3G modem, and has a fresh lemon scent #AGBT
+44m nanopore: of course! RT @kbradnam: @nanopore release also reveals that MinION syncs with iTunes, acts as a 3G modem, and has a fresh lemon scent #AGBT
+47m ING_SYS: Check out our poster: Biological Interpretation of NGS Re-sequencing Data- today 4:30pm to 6:00pm # 293 #AGBT
+53m howitts_done: Very cool technology! @pathogenomenick: Oxford Nanopore megaton announcement: Why do you need a machine? http://t.co/HydaHnmL #AGBT
+55m genetics_blog: Hmm. What will Oxford @nanopore's 4% error rate mean #bioinformatics? #AGBT
+55m Copenhagenomics: Anja introducing #CPHx 2012 from #AGBT - we hope to see you in Copenhagen, June 14-15 :) http://t.co/F1ExdRRx
+59m deannachurch: OK @nparmalee Carlos Bustamante is up now talking about Complete Genomics and population genetics inference in the personal era. #AGBT
+59m kbradnam: Things to tell your grandchildren: "and we used to work in large buildings called sequencing centers" #AGBT

3 - 4pm EST
+0m iontorrent: Yoga flash mob, informal 45 min, with Garrick @ 3:30 pm, Palms Lawn - Northern most lawn of the Marriott. Mats provided #namaste #AGBT
+1m deannachurch: CB: trying to address how many mutations do genomes from different populations carry + looking at ancient genomes and modern genomes. #AGBT
+1m deannachurch: CB: Comp. Genomics: average 55X coverage. Reference guided assembly + de novo assembly in variant regions. #AGBT
+2m deannachurch: CB: What can you find out from high coverage genomes that you miss with low coverage genomes? #AGBT
+2m ChaunceyGrattan: Can the pores sequence more than one fragment per run? @nanopores #AGBT
+3m mrfoyle: RT @rob_carlson Recalling that just two months ago several prominent biologists assured me #nanopore sequencing was fairy dust. Oops. #AGBT
+5m Awesomics: On the @Nanopore splash at #AGBT RT @matthewherper: The Next DNA Disruptor? - Forbes http://t.co/6ondWpl0
+5m illumina: Bustamante: CGI released 69 genomes avail as a reference and plans to do 500 more genomes #AGBT
+6m deannachurch: CB: Populations can vary in nucleotide diversity by two fold. #AGBT
+6m Copenhagenomics: Did the AV guy fall asleep? Bustamante is LOUD... #AGBT
+7m acdarby: I am a bit worried that my computer does not have enough USB ports to turn it into a sequencing lab #AGBT @nanopore!
+8m ldtimmerman: RT @Awesomics: On the @Nanopore splash at #AGBT RT @matthewherper: The Next DNA Disruptor? - Forbes http://t.co/vHisjesO
+9m deannachurch: CB: pop load of damaging homozygous mutations does not track w/ diversity. e.g. CHB low div. but higher rate of damaging mutations. #AGBT
+10m illumina: Bustamante: Genetic diversity among Chinese, Eurpoean, African pops. Excess diversity damaging? #AGBT
+11m bioinfosm: with so much #agbt buzz after the #oxford #nanopore talk, it might actually have become a trending topic on #twitter!!!
+11m deannachurch: CB: bottleneck followed by expansion allows for retention of deleterious mutations. Admixture is protective for hom. deleterious mut. #AGBT
+12m deannachurch: CB: Rest of talk not tweetable. #AGBT
+14m bkmacy: Nanopore genome sequencer makes its debut http://t.co/P7k0G5E0 @naturenews #AGBT
+14m omespeak: Somewhat lost in the hype of the MINIon is that the nanopore can potentially sequence RNA directly. Big news for RNA researchers. #AGBT
+14m illumina: Bustamante: Excess deleterious homoz muts characterized by >1 severe dramatic bottlenecks followed by population expansion #AGBT
+18m bkmacy: Can MinION by nanopore sequence RNA for gene expression investigations? #AGBT
+19m stveep: It's that time of year when I realise how "small potatoes" my sequencing projects are...but then why do they cause so much grief?! #AGBT
+20m Copenhagenomics: Bustamante: "Finns are special." <-- A return trip to Helsinki easily confirms that... ;) #scandinavianlove #AGBT
+21m saxscience: #AB471 MT @pathogenomenick: I've embedded all the #nanopore videos on the blog here http://t.co/wdAbG98h #AGBT
+22m leonidkruglyak: +1, and I'm not even at #AGBT No one is talking about much else right now. http://t.co/u2fQZhIM
+23m JasonDeKoning: Crazy stuff being announced at #AGBT! http://t.co/iDJ3V9E2
+24m bslatko: @agbt
+25m ayrrisbio: Why do you need a machine to do next-generation sequencing?http://t.co/wZIuV4Dk Lots more 'omics data coming!! #AGBT HT @Nanopore
+26m drgitlin: +2 RT @leonidkruglyak: +1, and I'm not even at #AGBT No one is talking about much else right now. http://t.co/7Kd3YPRG
+27m illumina: Watson: 1B reactions per day based on droplet streams #AGBT
+29m illumina: Watson: V. large number of singleplex PCR primers merged with gDNA in a droplet, then PCRd #AGBT
+30m froggleston: Rothberg: "Nanopores are an 'elegant' technology [..] but Ion Torrent already has a foot in the door" (NewSci). Palpable desperation? #agbt
+30m SarahSalbu: wow RT @finchtalk: #AGBT Kevin Hrusovsky on where PerkinElmer is going, mentions that 4th leading cause of death: drug side effects.
+39m GenomeBiology: Raindance discusses Olivier Harismendy's recent Genome Biology paper that used their technology #agbt
+40m illumina: #AGBT poster 191: Deep seq ribosome protected mRNA frags from poly- & monosomes isol by size excl chrom
+44m joe_pickrell: +1 RT @leonidkruglyak +1, and I'm not even at #AGBT No one is talking about much else right now. http://t.co/MZxSS5n4
+45m illumina: #AGBT poster 126: High accuracy and high speed sequencing on Illumina platforms
+45m parijata: RT @kbradnam: Reactions to @nanopore news. Biologists: cheer. Bioinformaticians: groan. System admins: weep. #AGBT
+45m EpicentreBio: RT @illumina: #AGBT poster 191: Deep seq ribosome protected mRNA frags from poly- & monosomes isol by size excl chrom
+46m NextBio: Visit us at #AGBT poster 370: Mining TCGA glioblastoma data for potential #biomarker! #PM101
+46m bioontology: RT @bioitworld: Full story on Oxford @nanopore stunning presentation from CTO Clive Brown at #AGBT http://t.co/5esocobA
+46m TrishWhetzel: RT @bioitworld: Full story on Oxford @nanopore stunning presentation from CTO Clive Brown at #AGBT http://t.co/0lpJgvWi
+46m bioontology: RT @bioitworld: Full story on Oxford @nanopore stunning presentation from CTO Clive Brown at #AGBT http://t.co/eE67nP5n
+50m illumina: #AGBT poster 234: #Nextera amplicon lib prep for Illuminas #MiSeq personal sequencing system
+54m drchriscole: @davidmam I'm not sure that'll happen. 100x coverage genomes will be unnecessary #ngs #nanopore #AGBT
+54m djschlesinger: Elaine Mardis talking about NuGen's microfluidics technology #AGBT
+55m illumina: #AGBT poster 149 Using #HiSeq2000 to detect de novo germline and somatic muts in clinical WGS
+56m rbuels: Huge, accurate, fast, super-cheap DNA reads? :-O Oxford #nanopore #ngs at #AGBT shot has definitely been heard round the world.
+58m EdgeBio: #AGBT @EdgeBio SAB chair Elaine Mardis up next to talk about NuGen microfluidics technology
+58m eonsim: I want some MinIon minions! Cheapish and disposable just as they should be! #agbt
+59m Massgenomics: Elaine Mardis on making libraries w/ 10-20 ng of DNA: "You gotta have Sean. But the bad news for you is, you can't have Sean." #AGBT.

4 - 5pm EST
+0m fluidigm: Only 60 minutes left! Stop by Suite 285 and learn how you can obtain reads from both ends with single-read sequencing. #AGBT ^S
+1m kbradnam: My #AGBT tweets have gained me several new followers today. Wonder how quick I'll lose them when I revert to tweeting about angry penguins?
+1m illumina: #AGBT poster 353: Single-day, highly multiplexed amplicon sequencing with #MiSeq
+1m illumina: Have a cocktail and meet our experts at Happy Hour in the Illumina Lounge (Capri Ballroom 2/3) now thru 7:00pm. #AGBT
+1m djschlesinger: If you haven't seen the NuGen system in person, it's pretty neat. Droplets move across the circuit board look like cartoon animation. #AGBT
+1m KamounLab: Oxford #Nanopores MinIon "run-until" option is cool. Great for sloppy labs. If wrong sample is loaded then stop the run #ngs #AGBT
+7m Massgenomics: Mardis: Low-input libraries @WashU: PCR cycle optimization helps control the duplication rate ==> better sequencing efficiency. #AGBT.
+9m suganthibala: AGBT 2012 Day 1: Clinical Annotation and Fierce Competition http://t.co/e9MMaa72
+9m razoralign: AGBTOxford Nanopore minIONw
+12m NEBiolabs: Meet NEB scientists at the #AGBT poster session at 4:30. We're at booth 256 talking about strand-specific RNA.
+13m Copenhagenomics: Here's a spy photo from a projected sequencing center, planned to open in early 2013: http://t.co/BjaSNe1P #AGBT
+15m lexnederbragt: Our competitors are like a pregnant woman" #ONT #AGBT http://t.co/hQHJY0at
+15m kbradnam: Heh! RT @Copenhagenomics: Here's a spy photo from a projected sequencing center, planned to open in early 2013: http://t.co/KcOFGxcZ #AGBT
+15m NEBiolabs: Meet NEB scientists at the #AGBT poster session at 4:30. We're poster 256 and talking about strand-specific RNA.
+17m schnablep: #AGBT #NGS game changer? press release from @nanopore: http://t.co/HC51JxFo more: http://t.co/FZoyImTP (do check these out)
+20m illumina: #AGBT Workshop: Illumina Targeted Reseq and Sample Prep Solutions -- starts in 10 min, Illumina Lounge (Capri Ballroom 2/3)
+20m daweonline: I'm not at #AGBT but I heard a couple of bombs falling!
+21m NEBiolabs: #AGBT poster session - visit us at poster 214 to learn about our library prep developments in adaptors!
+21m Matt_Francis: #AGBT obi-wan:I felt a great disturbance in the force, as if a million #illumina investors cried out in pain #oxford #Nanopore
+21m NeonAardvark: RT @bioinfosm with so much #agbt buzz after the #oxford #nanopore talk, it might actually have become a trending topic on #twitter!!!
+26m NEBiolabs: Come to Collier Hall and learn about #automation at poster 153 at the #AGBT poster session starting in 5 minutes!
+26m NeonAardvark: RT ILMN cheers storing genomes on a USB stick while Oxford #Nanopore was showing sequencing genomes WITH a USB stick? #AGBT #futureshock
+29m Calo_BM: #AGBT
+29m eperlste: @nparmalee +1! I've been on both sides of conference tweeting. Maybe it's the subject matter, but following #AGBT has been a blast.
+30m NEBiolabs: Chat with NEB scientists and learn about microbiome enrichment at poster 82 at the #AGBT poster session starting now in Collier Hall.
+33m EpicentreBio: Thursday afternoon at #AGBT: roundup from @Illumina http://t.co/NOYpw7qX
+36m kbradnam: Warning to all employees of @Nanopore. If you carry any 'regular' USB flash drives on you, prepare to be mugged by rabid genomicists #AGBT
+41m xprize: #AGBT RT @agxp: Head to poster 170 to meet Grant Campany Sr. Dir Archon Genomics X PRIZE presented by Medco. learn abt the $10M Competition
+42m emergentnexus: @MiriamGoldste There's also #AGBT where Oxford Nanopore announced USB stick sized ultra-long read $900 nanopore DNA sequencer #aaasmtg
+44m t_basi: I'm so relieved that's OVER. I don't understand any of it yet I've been on the edge of my seat all week. Well done Zoe @nanopore . #agbt
+46m eperlste: @sargoshoe #AGBT has generated the most scientific buzz I've seen on Twitter, and it has nothing to do with PLoS, arsenate or retractions!
+47m liu3zhen: Crazy world. I guess many PIs will re-write their proposals based on the unbelievable Nanopore new system #AGBT
+51m Jeylen: I plan to start up a sequencing center. :) MT @Copenhagenomics: a projected sequencing center in early 2013: http://t.co/9wvLMS1H #AGBT
+51m KazanLaw: I liked a @YouTube video from @iontorrent http://t.co/qFqVyIVX Follow the Ion Bus to AGBT 2012...Get On
+55m NEBiolabs: Visit NEB scientists at posters 82, 153, 214, and 256 at the #AGBT poster session going on now! http://t.co/z2Ex5VuN
+58m aindap: Twitter feed is exploding with @nanopore related #AGBT tweets!

5 - 6pm EST
+4m rkirkbride: Today, everyone was watching the race for the $1000 genome got blindsided by the $1000 genome sequenceER. #AGBT #nanopore
+5m illumina: Come on by for happy hour in the Illumina Lounge! #AGBT
+6m rkirkbride: Today Evonne who was watching the race for the $1000 genome got blindsided by the $1000 genome sequencER. #AGBT #minION
+13m bkmacy: RT @ING_SYS: Check out our poster NOW: Biological Interpretation of NGS Re-sequencing Data- today 4:30pm to 6:00pm #293 #AGBT
+17m nygenome: Lots of energy and interest in #nygenome at the #AGBT poster session. Stop by Collier #173 if you haven't visited us yet.
+17m nanopore: RT @kbradnam: Warning to all employees of @Nanopore. If u carry 'regular' USB drives on you, prepare to be mugged by rabid genomicists #AGBT
+18m sujaik: Keith Robinson adds a dash of realism to the #nanopore #agbt gushiness :-) http://t.co/AKwoNpbo
+19m bkmacy: At heart of the sequencing debate, researchers face the difficult task of relating data to affected biology http://t.co/ObGCOIR8 #AGBT
+21m genomesunzipped: Making sequencing simpler with nanopores: Our take on the new Oxford @Nanopore machines #AGBT http://t.co/cjrQX1db
+24m Clarksearch: U R Funny RT @kbradnam: Things to tell your grandchildren: "and we used to work in large buildings called sequencing centers" #AGBT
+24m GenomeInstitute: Details of Oxford Nanopore's Next Gen sequencing technology http://t.co/NkWv97NW (@bioitworld) #AGBT
+28m CompleteGenomic: Another exciting day at the #AGBT Complete Suite with our customers and partners. Hope everyone had a great time!
+31m JTBiotech: Crazy announcement at the #AGBT conference: #MiniION, a disposable USB pocket-sized nanopore sequencer: http://t.co/cg2JO6Xa
+34m rkirkbride: Everyone looking for $1000 genomes surprised today by $1000 genome sequencER. #AGBT #nanopore
+42m belvario: Entering the age of the $1K genome - http://t.co/RPVGsmMj and http://t.co/nXxdoeuf @nanopore @iontorrent #AGBT
+49m belvario: So, what is the exact use case for the @nanopore MinION? #AGBT
+52m coding_doc: @belvario Have look at the @GenomesUnzipped blog http://t.co/gkPdIm14 #AGBT
+52m mjcgenetics: Oxford @Nanopore's MiniON. A DNA sequencer the size of a memory stick! Put away that tricorder, Bones. http://t.co/ZTbMe5Od #AGBT #genomics
+53m Copenhagenomics: We sure are minions of the MinION... Thanks for all the retweets :) #AGBT
+54m neilhall_uk: "Its like youve been in a band. doing gigs in grotty little venues,then you wake up and youre number one" http://t.co/GQr4acRW #ONT #AGBT

6 - 7pm EST
+3m belvario: Anyone else thinking GATTACA? RT @coding_doc: @belvario Have look at the @GenomesUnzipped blog http://t.co/E35JYGeK #AGBT
+5m kbradnam: If you're going to get a MinION then you may as well buy one of these as well (http:/worldssmallestpc.com/ultrasmall.htm) #AGBT #smallworld
+6m DonRosenthal: MT @westr @genomesunzipped: Making sequencing simpler w/ nanopores: Our take on the new Oxford @Nanopore machines #AGBT http://t.co/IPrsp8Zt
+19m Personal_RX_WI: #AGBT Not a bad place to spend 4 days. Thanks AGBT! http://t.co/Z2eKHkT8
+28m genomematt: New announcement from oxford @nanopore are the talk of the sequencing world after #agbt announcements http://t.co/8kDyKQOd
+29m 8iterations: Rack-mounted USB nanopore DNA sequencers RT @Copenhagenomics: spy photo : http://t.co/Eyz3RMxf #AGBT
+31m HRSearchPros: Are you a good fit for this job? Recruiting Manager in Houston, TX http://t.co/KyymMXgs #job
+32m assemblathon: RT @genomesunzipped: Making sequencing simpler with nanopores: Our take on the new Oxford @Nanopore machines #AGBT http://t.co/SZmpwcbz
+32m GenomeWeb: Impact of @nanopore's #minIon announcement on rest of sequencing market is pretty telling: http://t.co/GCB4Up9l #AGBT
+33m assemblathon: How long before a MinION is featured on CSI? Though it would of course need a blue light and be able to sequence a genome in seconds. #AGBT
+35m kbradnam: Of course @nanopore are having the last laugh as they haven't told people that the MinION syncs data over USB 1.0 #AGBT
+43m TrishWhetzel: RT @genomeweb: Impact of @nanopore's #minIon announcement on rest of sequencing market is pretty telling: http://t.co/wlg0kWvY #AGBT
+47m kb_macrolee: $1000 genomes in 2013 ? RT @genomesunzipped: simpler with nanopores: on the new Oxford @Nanopore machines #AGBT http://t.co/XFXn9Pln

7 - 8pm EST
+2m becky_furlong: looks like I should have gone to #agbt just for the @nanopore talk ...
+8m humanlifee: AGBT 2012 Tweets Day 3: 9:00-9:35 am, Keynote Speaker: Rick Myers, HudsonAlpha Institute for Biotechnology, Gen... http://t.co/6F3Aclqd
+8m aninditaarci: AGBT for ...
+9m IanGoodhead: I feel sorry for anyone else giving a talk today at #AGBT.
+12m belvario: Everyone's focusing on the price/speed - my money's on the real story being long reads... @nanopore #AGBT
+18m Symbionticism: Oxford nanopore usb-sequencer is like bringing social media to genomicists. We will all be empowered instead of waiting for cores. #AGBT
+29m deannachurch: Getting ready for @girlscientist to talk in the Genome Biology session tonight: measuring T cell repertoire diversity. #AGBT
+31m chloe46037: Chris gunter is up #AGBT "measurement of TCell repertoire diversity
+31m genomeresearch: The fabulous Chris Gunter from HudsonAlpha talking about T cell repertoire diversity at #AGBT
+32m deannachurch: CG: focusing on the beta receptor- looking to measure genetic and environment interaction #AGBT
+33m 613SportsChick: I need to tweet as @girlscientist is presenting at #AGBT right now! #tweetfriendlytalk
+34m deannachurch: CG: Looking at the diversity using RNA rather than DNA. mostly sequencing on 454 but also some Illumina. #AGBT
+34m iontorrent: Great AGBT talk by Dr. Tim Triche about long-read RNA Seq on the Ion PGM...: http://t.co/eCJbdXV9 via @youtube #agbt #ionbus
+35m deannachurch: CG: looking at repeatability (looks good) and quantitation (semi-quantitative). #AGBT
+36m ElementoLab: #AGBT Scott Grimmond: genomic analysis of pancreatic adenocarcinoma
+36m chloe46037: CG:multiplexing PCR primers in the TCRB genes #AGBT.
+37m genomeresearch: related http://t.co/vQRlXuBF RT @deannachurch: CG: focusing on the beta receptor-looking to measure genetic & environment interaction #AGBT
+38m bffo: CG cool visualization tools .#agbt
+38m deannachurch: CG: Able to see difference in TCRB between normal samples and both Lupus and colon cancer samples. #AGBT
+38m bffo: Chris Gunter (.@girlscientist) talks about cool stuff.! #agbt
+41m GenomicsIo: MT @belvario: [] - my money's on the real story being long reads... @nanopore #AGBT & RNA & protein & small molecue-seq on same hardware
+41m deannachurch: CG: advertising the "R10K" project- want to sequence the immune repertoire of 10,000 individuals, 100 each and 100 diseases. #agbt
+41m salisburymw: Full house for the @girlscientist talk on immune response. Very cool work. #agbt
+41m coding_doc: RT @genomeresearch: The fabulous @girlscientist from HudsonAlpha talking about T cell repertoire diversity at #AGBT
+41m deannachurch: CG: go to http://t.co/rekf2Gkd for more information on joining the project! #AGBT
+43m ElementoLab: #AGBT Scott Grimmond: DNA damage repair genes often mutated in PDAC .. also includes p300
+43m GenomicsIo: MT @iontorrent: AGBT talk by Dr. Tim Triche about long-read RNA Seq on the Ion PGM.: http://t.co/AjQLr5Vd via @youtube #agbt #ionbus thx!
+44m obahcall: @girlscientist speaking on http://t.co/GWcVJCOQ , sequence immune repertoire in 10k, 100 each in 100 diseases #AGBT
+44m JChrisPires: FREEDOM! fancy new OxNano pocket sequencers #sequencing has finally broken free of the lab! #genome #bioinformatics #AGBT
+45m JChrisPires: RT @bffo: CG cool #visualization tools #bioinformatics #agbt
+46m ElementoLab: #AGBT Scott Grimmond: axon guidance genes eg Robo2, Slt2, Sema3A often mutated in pancreatic adenocarcinoma
+47m jewmanchue: @bmooreUT @foryvonne @genotyper: Congrats! Major #VAAST twitter activity from #AGBT including: @illumina @iontorrent #YandellLab #FTW
+48m jewmanchue: @illumina FYI its Lynn Jorde; not Lynne :-) #VAAST #AGBT
+48m obahcall: Joanna Kelley on multi platform de novo assembly of self fertilizing hermaphroditic fish #AGBT
+48m ElementoLab: #AGBT Scott Grimmond: ROBO2 and ROBO3 expression correlate significantly with survival in pancreatic adenocarcinoma
+50m CaitieL: "game changer" new sequencer revealed at #AGBT - thoughts? http://t.co/IeEyjnc6
+51m deannachurch: Joanna Kelley talking about sequencing and de novo a self-fertilizing hermaphroditic fish. #AGBT
+51m froggleston: No tweets about surname leakage. A tad ironic one could say. #agbt
+53m jewmanchue: @GholsonLyon Dr. Jorde shared the NAA10 story at #AGBT
+53m obahcall: Kelley: multi-platform approach de novo genome sequence of self fertilizing hermaphroditic fish ... We are literally swimming in data #AGBT
+54m ElementoLab: #AGBT David Smith: Exome, Transcriptome and Methylome Analysis of Oropharygneal Cancers
+54m mgberlin: @nanopore Why not set up with a minION and a laptop in the corner of #AGBT and sequence genomes? All promises, no proof.
+56m rnomics: RT @GenomicsIo: MT @iontorrent: AGBT talk by Dr. Tim Triche about long-read #RNA Seq on... http://t.co/2OHsXK34 #rnomics-bioscience @MyEN
+57m bioinfosm: multiple Informatics Specialist job openings at Mayo Clinic http://t.co/kkuFWskx
#agbt #jobs #ngs
+58m jgreid: AGBT Agilent Party http://t.co/n5M4nkqp
+59m girlscientist: Thanks all for the tweets of the talk! Main message: we welcome you to participate in the R10K project, http://t.co/Cj2Qcz0s #AGBT
+59m Massgenomics: David Smith (Mayo) on oral pharynx carcinoma: Some exomes, some RNA-seq, some methylome. Talking so fast I can't write anything down. #AGBT

8 - 9pm EST
+2m genome_gov: Kelley: will use RNASeq to improve assembly in future work #AGBT
+2m obahcall: Kelley: discussing challenges to taking mixed approach, mainly bioinformatics, some assemblers not handle ion torrent data. #AGBT
+3m genome_gov: Kelley: population genomic analysis shows highly homozygous populations #AGBT
+3m girlscientist: Like being attacked by dementors, eating chocolate helps after giving an #AGBT talk.
+5m girlscientist: Joanna Kelley did a great job talking about fish genome sequencing and problems with trying to combine data from multiple platforms. #AGBT
+5m Massgenomics: David Smith: No differences in somatic mutation count between current smokers (HPV-) and never-smokers (HPV+). Doesn't make sense. #AGBT
+5m lykkebak: #AGBT Interesting talk on smokers/non smokers/former smokers by David Smith. Still hope for the smokers - if they stop smoking.
+6m genome_gov: Martin Hirst: Epigenetic contributions to cell identity in human breast #AGBT
+10m ElementoLab: #AGBT John McPherson: Evaluating Targeted Resequencing as a Diagnostic Tool: a Prospective Clinical Trial
+11m obahcall: John McPherson on targeted resequencing as a cancer diagnostic tool #AGBT
+12m girlscientist: Martin Hirst: "I would argue one of seminal findings of RNA seq-ing in cancer are the recurrent findings of epigenetic modifiers." #AGBT
+13m chloe46037: John McPherson "evaluating targeted resequencing as a diagnostic tool" #AGBT
+16m jewmanchue: hey #AGBT folks: do you have a link to @nanopore summary statistics? #depth #coverage #quality?
+16m girlscientist: Hirst has asked us not to tweet the details of his breast cancer epigenetic data, sorry. #AGBT
+16m Massgenomics: Hirst on breast cancer epigenetics. Epigenetic modifiers recurrently mutated: MLL2, ARID1A examples. Ever heard of DNMT3A, guy? #AGBT.
+17m Massgenomics: Well I guess I asked for it - Hirst just asked for "no tweeting" at #AGBT. Rarely a good sign for a talk, ironically.
+20m chloe46037: JMP reports to clinician composed of mutation, frequency and clinical studies relevant to reported mutations #AGBT
+27m illumina: Hirst: Many cancers associated with genome hypomethylation and paradoxically CpG island hypermethylation #AGBT
+28m genome_gov: Alan Harris on Evolutionary genetics of small body size and dizygotic twinning in Callitrichine Primates #AGBT
+28m girlscientist: Awwww...Alan Harris of Baylor showing cute monkeys. #AGBT
+29m girlscientist: [except that one of the small monkeys in Harris's slide looks like he has wicked teeth and he is gouging a tree to get the bark off. #AGBT]
+30m girlscientist: Weird. Harris: these callitrichine primates have dichorionic, dizygotic twins 80% of wild births. Twins all the time: dream genetics! #AGBT
+30m ElementoLab: #AGBT John McPherson: reported cancer diagnostic testing results of 19 actionable genes, using PacBio and Sequenom on 30 patients
+31m obahcall: Samuel Levy: somatic detection in tumor genomes using Illumina and Ion Torrent seq data #AGBT
+33m ElementoLab: #AGBT John McPherson: on 19 gene panel, PacBio found a few more actionable mutations than Sequenom, because entire genes were sequenced
+33m Massgenomics: Levy: Assembly-to-assembly comparison can reveal complex DNA variation, i.e. as found in Craig Venter's genome. Ah, name-dropping. #AGBT.
+33m neandrothal: @dgmacarthur We haven't seen the real product yet. PacBio gave similar predictions at AGBT before
+37m Massgenomics: Levy: De novo Assembly Assembly Mapping (DAAM). Wins the acronym award, at least. #AGBT.
+38m girlscientist: You're at #AGBT? And you're reading twitter? You should meet the other tweeps in person. Hotel lobby bar, when sessions end.
+38m ElementoLab: #AGBT Samuel Levy: using an assembly approach to look for indels (instead of simply aligning reads using short read aligners)
+39m ElementoLab: #AGBT Samuel Levy: assembly approach is called DA-AM, detects 2-10 fold longer indels than GATK
+41m BiotechMarketer: I absolutely love being able to attend #AGBT vicariously via twitter. Thanks to all the tweeps at Marco Island!
+41m Massgenomics: The DAAM approach calls them somatic. The DAAM approach ends up producing stuff that's more accurate. I'll be DAAMed. #AGBT.
+43m ElementoLab: #AGBT Samuel Levy: showed a nice 15bp somatic insertion missed by GATK (functionality unclear since it is in 5'UTR of a LOC gene)
+44m Massgenomics: Levy: 96-97% mapping rate for IonTorrent 200-bp reads. DK: But let's be honest: 2x coverage is a far cry from validation. #AGBT.
+44m bigfeet5grl: 10Kbp > 40bp reads @belvario: Everyone's focusing on the price/speed - my money's on the real story being long reads... @nanopore #AGBT
+46m spbccar: #Agbt push button perfect bacterial assemblies from david jaffe using allpaths-lg
+48m ElementoLab: #AGBT Rebecca Kusko: comprehensive profiling of lung transcriptome in COPD and IPF patients using RNA-seq
+53m girlscientist: Michael Talkowski: importance of balanced chromosomal rearrangements in autism. Sorry but I didn't catch whether talk tweetable. #AGBT
+53m genome_gov: Michael Talkowski on Balanced chromosomal rearrangements in Autism and other neurodevelopmental disorders #AGBT
+54m thinkCara: I so want to go to a scientific conference with a hashtag. But alas, I'm a first year grad student so I must wait. #AGBT
+56m froggleston: Flagging. Need beverages. #agbt
+57m benoitbruneau: Transcripts, assembly RT@belvario: Everyone's focusing on the price/speed - my money's on the real story being long reads... @nanopore #AGBT
+57m genomeresearch: Original Allpaths http://t.co/AzC6Plgf RT @spbccar: #Agbt push button perfect bacterial assemblies from david jaffe using allpaths-lg
+58m NJL_Broad: David Jaffe of @broadinstitute : near perfect assemblies of microbes by combining illumina fragments, jumps and pacbio long reads. #AGBT

9 - 10pm EST
+0m spiremk: Just absorbing the #AGBT @nanopore talk from earlier today. Screw the #iPhone introduction, this is truly mind-blowing http://t.co/J2vupPcE
+1m ElementoLab: #AGBT Rebecca Kusko: most interesting finding is wound healing cytokine alternatively spliced in IPF and emphysema
+2m genome_gov: Talkowski: chromothrpsis = chromosomal shattering and rearrangement #AGBT
+5m froggleston: Potential use of @nanopore for gut microbiomes? Glad those USB sticks are throw away. @CrapBio takeover imminent? #agbt
+7m girlscientist: Talkowski's work is pretty cool, on chromothripsis in autism. More on him at http://t.co/h5sltduP #AGBT
+7m djschlesinger: Anyone going to the Ion Bus party? #AGBT
+10m deannachurch: Getting ready for @NCBI 's Eugene Yaschenko's talk on compression strategies for NGS data. #AGBT
+11m girlscientist: Crowd filing back into room and standing in back for Eric Schadt talk next. Modestly titled on slide, "Reveal the true biology." #AGBT
+12m ElementoLab: #AGBT Maria Mendez-Lago: Genomic analysis of DLBCL
+12m genome_gov: Eric Shcadt on Kinetic Variation events, SMRT sequencing and novel regulatory mechanisms #AGBT
+13m obahcall: Eric Schadt on novel regulatory mechanisms from more complete characterization DNA methyl #AGBT
+14m deannachurch: EY: Going over decision circle needed for defining compression: what to store, remove redundancy, lossy vs. lossless, tuning. #AGBT
+14m girlscientist: Strap in people: Schadt starting on real-time pathogen outbreak studies with @pacbio. First: summer 2011 E. coli outbreak in Germany. #AGBT
+14m genome_gov: Schadt: E.coli outbreak in Germany; why was it so virulent? #AGBT
+16m girlscientist: Schadt: got 8 E. coli genomes of strain to compare, and can see outbreak strain only has shiga toxin gene addition. Explains pathology #AGBT
+16m genome_gov: Schadt: phagelike element encoding shiga toxin found in outbreak strain #AGBT
+16m obahcall: Schadt reviews their studies on pathogen sequencing during epidemic outbreaks, first on Ecoli outbreak in Germany July 2011 #AGBT
+17m genome_gov: Schadt: horizontal gene transfer indicated in the increased virulence in outbreak strain #AGBT
+18m girlscientist: Schadt: sequence data led them to hypothesize that some antibiotics would make symptoms much worse; held up with data. #AGBT
+19m deannachurch: EY: need to update SRA compression to accommodate BAM and other formats containing both raw reads and alignments. #AGBT
+20m pyknosis: RT @kbradnam: Reactions to @nanopore sequencer news. Biologists: cheer. Bioinformaticians: groan. System admins: weep. #AGBT
+20m deannachurch: EY: describing update of cSRA toolkit- you can get this software here: http://t.co/9KWUl5G3 #AGBT
+20m girlscientist: Schadt: now to add methylation data. Going through methods of 5meC detection on @pacbio. #AGBT
+20m genome_gov: Schadt: using PacBio to detect base modifications #AGBT
+22m girlscientist: Schadt: see massive signature of methylation on outbreak genome compared to reference E. coli strain. What are modfications doing?? #AGBT
+22m djschlesinger: Do you have all your Ion tiles?? #AGBT http://t.co/EUX8EcTR
+22m deannachurch: EY: testing new compression method on 1000G data- over 3114 files taking 27 Tb of data after compression. #AGBT
+23m obahcall: Schadt: now reanalyzing this Ecoli outbreak seq data using @PacBio to ID kinetic variation events #AGBT
+25m girlscientist: Schadt: found completely novel sequence contexts for adenine methylation in this outbreak strain. #AGBT
+28m girlscientist: Schadt: then use RNA-seq to look for enrichment of pathways in outbreak strain. Genes upregulated likely to have new motif, modified. #AGBT
+29m girlscientist: Schadt: now giving us an animated summary of his entire talk, with videos. #AGBT Guess I shouldn't have worried about Prezi being too fancy.
+29m deannachurch: EY: Updates to SRA Run browser to add additional information and to make it easier to show alignment. #AGBT
+29m myopomme: Sequencing Takes a Clinical Bent at AGBT http://t.co/Z8VPpukx We can follow the NGS meeting. tech, application, and clinical use. #AGBT
+30m deannachurch: EY: New tool to make access to 1000 Genomes data (BAMs and genotypes)- including downloading BAM slices: http://t.co/9KWUl5G3 #AGBT
+31m girlscientist: Whew. The tweetup should begin shortly. #AGBT
+34m omespeak: Already here... RT @girlscientist Whew. The tweetup should begin shortly. #AGBT
+36m 454Sequencing: Don't miss the Roche Applied Science Workshop tomorrow at noon in the Palms Ballroom! #AGBT
+43m ramoyw2: AGBT
+45m 454Sequencing: "A draft assembly of Cannabis saliva and Cannabis indica" Presented by Kevin McKernan, Medicinal Genomics - Don't miss it! #AGBT
+54m Massgenomics: Day 2 summary of #AGBT and cool photo posted on Massgenomics: http://t.co/APPtxVay
+56m jgreid: @AGBT IonLounge http://t.co/gJTVd6lB
+57m ElementoLab: #AGBT Maria Mendez-Lago: found novel fusions including w FOXP1, also novel targets of aberrant somatic hypermutation in DLBCL, many overexp

10 - 11pm EST
+6m liu3zhen: A great AGBT notes; get information without attending the conference http://t.co/ueTd1A72 #AGBT
+15m kbradnam: A crazy day of #AGBT-related tweets. Hello to my new followers...I'm sure you will be unfollowing me within a week!
+24m bffo: at bar, waiting for tweet up ... middle table ... #agbt
+34m dgmacarthur: Great coverage of #AGBT by @Massgenomics: http://t.co/Hv3aZdSz
+35m girlscientist: Massive turnout for tweetup! Feeling the love. #AGBT
+44m Dr_Bik: @kbradnam are you at #AGBT IRL or just following online? Sounds cool either way!
+51m liu3zhen: MinIon can be used to QC your DNA libraries. Whatever libraries. That is great. #AGBT

11 - 12pm EST
+1m sciolato: So cool! RT @pathogenomenick: I've embedded all the nanopore videos on the blog here http://t.co/mD6XZINx #AGBT
+32m SEQanswers: Thanks to @nanopore for driving a subset of dummies out of $ILMN!! #AGBT
+43m caddymob: Is this still a thing #AGBT? "Illumina and Oxford Nanopore Enter into Broad Commercialization Agreement" http://t.co/IPWdRYv6
+45m hiro_h: AGBT tweets 2012 (2) http://t.co/8YWvx4S9
+46m TogetterJP: :

@hiro_h
AGBT tweets 2012 (2)

fav:0 view:0
http://t.co/WtUd5zah
+10m ribozyme: Also this: "Illumina Joins $41M Bet on Oxford Nanopore" http://t.co/V84gR201 RT @caddymob: Is this still a thing?. #AGBT
+17m fionabrinkman: Wow MT @pathogenomenick Oxford Nanopore megaton announcement: Why do you need a machine? interview http://t.co/8br4kwtb #AGBT
+29m EvgenyGlazov: Video: Dr. Tim Triche talks about long-read ncRNA Seq on the @iontorrent PGM and transcriptional complexity at #AGBT http://t.co/4ubDsVPo
+31m terryvrijenhoek: RT@nanopore: Introducing the MinION, a nanopore sequencer the size of a USB stick. Works just like GridION, but smaller #AGBT
+29m drsteventucker: "We wander into the realms of sci-fi at this point." @pathogenomenick interview with @nanopore #AGBT http://t.co/uIoDGvNa
+34m gantengp2: AGBT
+47m Arabgenes: Rt @nanopore Introducing the MinION, a nanopore sequencer the size of a USB stick. Works just like GridION, but smaller #AGBT
+49m KamounLab: @AnneOsterrieder @ThePlantJournal @MPPjournal @PetraKiviniemi @PlantBizarre Easy: Ask them to check hashtags #AGBT #LGD2012 #FRA2012 etc.
+50m Arabgenes: Rt @genetics_blog If looking through the AGBT tag is too daunting,@pathogenomenick is doing a fine job summarizing at http://t.co/Py4R8uA8.
+1m jessfl4: AGBT
+6m flyembtn9: HiSeq 1500 is same as 2500 with single lane flow cell. AGBT - what's the point?
+37m Hyoobaloinz: RT @genomesunzipped: Making sequencing simpler w nanopores: Our take on the new Oxford @Nanopore machines #AGBT http://t.co/6WMW7GfV
+43m mrrizkallah: Iit's been 2 hours and I am half way #AGBT tweets. This is a 500X coverage :-)